泥鳅蛋白多肽的抗氧化活性

Study on Antioxidant Activity of Loach Protein Polypeptide

为进一步促进泥鳅蛋白及其加工食品的开发,通过体内以及体外抗氧化实验,对泥鳅蛋白多肽抗氧化能力进行了研究。实验表明,泥鳅蛋白多肽在体外具有一定的抗氧化能力。体内抗氧化实验中,多肽高剂量组中血清、肝脏、心脏的SOD活性与模型对照组相比分别提升36.6%、28.1%、22.3%,GSH-Px活性分别提升43.2%、132.2%、92.5%,MDA水平分别降低了16.2%、18.3%、26.2%。实验说明,泥鳅蛋白多肽具有提高小鼠血清、肝脏以及心脏组织SOD活性以及GSH-Px活性,同时能够降低组织MDA水平的作用。肝脏组织切片表明泥鳅蛋白多肽对降低小鼠肝脏因氧化应激而产生的损伤具有一定效果。实验结果说明泥鳅蛋白多肽一定程度上可抑制氧化造成的损伤,并提升机体的抗氧化能力,对泥鳅蛋白及其深加工产品开发提供了参考。

In this paper,the antioxidant capacity of loach protein polypeptide was studied by antioxidant experiments.Experiments showed that loach protein polypeptide in vitro has a certain ability to antioxidant.In the antioxidant experiment in vivo,SOD activity in high-dose group was increased by 36.6%,28.1% and 22.3%,GSH-Px activity was increased by 43.2%,132.2% and 92.5%,respectively,while MDA level was decreased by 16.2%,18.3% and 26.2%,respectively.The experiment showed that loach protein polypeptide could improve the activities of SOD and GSH-Px in serum,liver and heart tissue of mice,and reduce the level of MDA in tissue.Liver tissue section showed that loach protein polypeptide had a certain effect on mice liver from oxidative stress damage.The experimental results showed that loach protein polypeptide could inhibit the damage caused by oxidation to a certain extent,and improve the antioxidant capacity of the body.The results provided reference for loach protein and its deep processing products.