氟苯尼考对水生细菌耐药性的影响研究

Effect of Florfenicol on the Drug Resistance of Aquatic Bacteria

[目的]建立水体模型,探究氟苯尼考对水生细菌耐药性的影响。[方法]将水体样品分成Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组,每组3个重复,各组添加不同浓度的氟苯尼考。分离培养建模前以及建模后第14、28、56天水生细菌,利用微量肉汤二倍稀释法和KB药敏纸片法对分离的水生细菌分别进行氟苯尼考和17种抗菌药物的敏感性试验。[结果]第56天收集的Ⅴ组水生细菌对氟苯尼考的耐药率最高,达到25.00%;分离的氟苯尼考敏感菌和耐药菌对阿莫西林、链霉素和多西环素等8种药物的敏感性差异极显著(P<0.01)。[结论]氟苯尼考在一定程度上增加了水生细菌的耐药性。

[Objective]To establish a water model and study the effects of florfenicol on the drug resistance of aquatic bacteria.[Method]The water samples were divided into groupsⅠ,Ⅱ,Ⅲ,ⅣandⅤwith 3 replications in each group,and different concentrations of florfenicol were added.The sensitivity tests of florfenicol and 17 kinds of antibiotics were carried out on the aquatic bacteria isolated and cultured before modeling and on the 14^(th),28^(th) and 56^(th) days after modeling by double dilution method of broth and KB disk method.[Result]On the 56^(th) day,the resistance rate of aquatic bacteria in group V to florfenicol was the highest(25.00%).The sensitivity of florfenicol sensitive bacteria and drug-resistant bacteria to 8 kinds of drugs(amoxicillin,streptomycin,doxycycline,etc.)was extremely significantly different(P<0.01).[Conclusion]Florfenicol increased the drug resistance of aquatic bacteria to a certain extent.