健康与患根腐病草莓根际·非根际与根内古菌群落多样性

Archaea Community Diversity in Rhizosphere, Non-rhizosphere and Root Tissue of Healthy and Root Rot Strawberry Plants

[目的]分析健康与患根腐病草莓根际土壤、非根际土壤及根内古菌群落结构与多样性。[方法]运用Illumina MiSeq高通量测序技术,分别对根际土壤、非根际土壤及根内古菌16S rRNA基因的V3~V4区进行扩增并测序,分析健康与患根腐病草莓不同部位古细菌群落结构与多样性。[结果]健康组共鉴定出11个门、19个纲、25个目、29个科、43个属,患根腐病组共鉴定出11个门、20个纲、27个目、32个科、47个属。门水平上,所有土壤样品的优势古菌门均为奇古菌门(Thaumarchaeota)、广古菌门(Euryarchaeota)和乌斯古菌门(Woesearchaeota),所有根内样品优势菌门均为奇古菌门和广古菌门;患根腐病草莓根际和非根际土壤的奇古菌门相对丰度均低于健康草莓,广古菌门和乌斯古菌门相对丰度均高于健康草莓,患根腐病草莓根内样品的奇古菌门和广古菌门相对丰度均低于健康草莓。属水平上,所有土壤样品的优势属均为亚硝化球菌属(Nitrososphaera)、亚硝化侏儒菌属(Nitrosopumilus)和Methanomassiliicoccus属,患根腐病草莓根际土壤亚硝化球菌属相对丰度比健康减少9.27百分点,非根际土壤亚硝化球菌属相对丰度比健康减少9.87百分点;所有根内样品的优势属均为亚硝化球菌属和甲烷孢菌属(Methanocella),亚硝化球菌属相对丰度比健康增加15.13百分点。[结论]健康与患根腐病草莓根际土壤、非根际土壤及根内样品古菌群落组成差异明显,说明草莓根腐病的发生与根际土壤、非根际土壤以及根内古菌群落结构改变密切相关。

[Objective]To analyze the archaea community structure and diversity of healthy and root rot strawberry in rhizosphere soil,non-rhizosphere soil and root tissue.[Method]The V3-V4 region of the archaea 16S rRNA gene in rhizosphere soil,non-rhizosphere soil and root tissue was amplified,and the amplified fragments were sequenced by Illumina MiSeq high-throughput sequencing technology.[Result]Healthy and root rot strawberry group samples got 11 phyla,19 classes,25 orders,29 families,43 genera and 11 phyla,20 classes,27 orders,32 families,47 genera,respectively.Thaumarchaeota,Euryarchaeota and Woesearchaeota were the dominant phyla in all soil sample,Thaumarchaeota and Euryarchaeota were the dominant phyla in allroot tissue.Compared with healthy strawberry plants,the relative abundance of Thaumarchaeota inrhizosphere and non-rhizosphere soil of root rot strawberry plant was generally lower,Euryarchaeota and Woesearchaeota was generally greater.The relative abundance of Thaumarchaeota and Euryarchaeotain root tissue of root rot strawberry plant was generally lower than healthy plants.The dominant genera in all soil samples were Nitrososphaera,Nitrosopumilus and Methanomassiliicoccus.Nitrososphaera and Methanocella were the dominant genera in all root tissue samples.Compared with healthy strawberry plants,the relative abundance of Nitrososphaera in rhizosphere and non-rhizosphere soil was 9.27 and 9.87 percentage point lower,respectively,which in root tissue was 15.13 percentage point higher.[Conclusion]There were significant differences in archaea community composition of rhizosphere soil,non-rhizosphere soil and root tissue from healthy and rootrot strawberry.Occurrence of strawberry rootrot was closed to thevariation ofarchaea community structure in field soil and root tissue.