几种鱼类胶原蛋白营养生理的初探

Preliminary Study on Nutritive Physiology of Several Fish Collagen Proteins

[目的]对云南昆明本地饲养的9种鱼类胶原蛋白营养生理进行分析和评估。[方法]选取9种鱼类,对其进行粗蛋白和胶原蛋白含量测定,并挑选4种食性不同的鱼类进行col1a1基因表达水平测定。[结果]9种鱼类粗蛋白含量以加州鲈最高(占全鱼总重的20.84%),鳜鱼次之(占比为20.10%);鱼类胶原蛋白含量最高的为裂腹鱼(7.65μg/g),鳙鱼次之(6.58μg/g)。4种鱼类中,col1a1基因表达水平最高的为裂腹鱼,且与其他3种鱼类的表达水平间差异显著(P<0.05)。[结论]该研究可为实际生产过程中胶原蛋白原材料的选取提供理论参考。

[Objective]To analyze and evaluate the nutritional physiology of collagen in 9 species of fish raised locally in Kunming,Yunnan Province.[Method]The content of crude protein and collagen in 9 species of fish were determined,and the expression level of col1a1 gene in 4 species of fish with different diets was determined.[Result]The crude protein content of california perch was the highest(20.84%of total body weight),followed by mandarin fish(20.10%).The highest collagen content was found in schizobelly fish(7.65μg/g),followed by bighead fish(6.58μg/g).The highest expression level of col1a1 gene was found in schizobelly fish,and there was significant difference between the expression level of col1a1 gene and the other three species(P<0.05).[Conclusion]This study can provide a theoretical reference for the selection of collagen raw materials in the actual production process.