基于碳纳米点荧光试纸可视化探针的构建及应用

Construction and Application of Visual Sensor Based on Carbon Nanodots Fluorescent Test Paper

以吩噻嗪为原料,经硝化反应合成出3,7-二硝基吩噻嗪,并以此为前驱体,制备出两种N、S掺杂的碳纳米点材料,通过多种手段对所合成材料结构和组成进行表征。将碳纳米点应用于Cu^(2+)的快速定性/定量可视化检测,并基于碳纳米点荧光试纸结合智能手机的颜色分析识别,构建Cu^(2+)可视化检测传感器,展现出高效、准确、灵敏和便捷的优势。在0~5μmol/L范围内的碳纳米点荧光差值和Cu^(2+)浓度的相关系数R 2=0.9976,Cu^(2+)检出限可达0.26μmol/L;在0~15μmol/L范围内,试纸荧光颜色的B/R值与Cu^(2+)浓度呈现较好的线性关系,相关系数R^(2)=0.9213,Cu^(2+)检出限可达1.28μmol/L。该荧光试纸的开发和应用为功能化碳纳米点材料提供了有益思路,并有望应用于现场和实时检测中。

Two N,S-co-doped carbon nanodots(CNDs)were prepared by using 3,7-dinitrophenylthiazine as precursor derived from nitration of phenothiazine,and were characterized for the structures and compositions by several techniques.The obtained CNDs were applied to the rapid qualitative,quantitative visualization detection of Cu^(2+)and to the fabrication of CNDs-based fluorescent test paper for smart phone′s color analysis and recognition.Such constructed Cu^(2+)visual detection sensor was corroborated to be effective,accurate,sensitive and convenient.The fluorescence difference of CNDs and Cu^(2+)concentration in the range of 0~5μmol/L showed a good linearity,the correlation coefficient R^(2)=0.9976,and the detection limit reached 0.26μmol/L.In the range of 0~15μmol/L,the B/R value of fluorescent test paper exhibited a good linear relationship with Cu^(2+)concentration,the correlation coefficient R^(2)=0.9213,and the detection limit of Cu^(2+)reached 1.28μmol/L.The development and application of the test paper provides a useful idea for functionalized CNDs materials,and is expected for application in field and real-time detection.