五种杀菌剂胁迫下灰葡萄孢产孢所需碳源种类分析

Carbon sources analysis for conidia formation of Botrytis cinerea under pressures of five fungicides

灰葡萄孢是引起作物灰霉病的病原菌,分生孢子是其传播的主要载体。本文采用代谢技术分析了灰葡萄孢对Biolog FF板碳源的利用及其产孢情况,并测定了在多菌灵、丙环唑、嘧霉胺、异菌脲和咪鲜胺5种杀菌剂胁迫下灰葡萄孢产孢所需碳源种类。结果表明:糖类、氨基酸类等92种碳源均能被灰葡萄孢代谢,其中,杏仁苷、L-阿拉伯糖等35种碳源能促进其分生孢子的形成;吐温80、D-阿拉伯糖、葡萄糖醛酸等57种碳源能被其代谢,但不能促进其分生孢子形成。用多菌灵1 mg/L、咪鲜胺5 mg/L或丙环唑1和10 mg/L处理,均可减少灰葡萄孢产孢所需碳源种类;而用嘧霉胺0.08和1 mg/L、异菌脲0.1和5 mg/L处理,对灰葡萄孢产孢所需碳源种类均无明显影响。相关研究结果揭示了在杀菌剂胁迫下灰葡萄产孢所需碳源种类,可为灰霉病化学防控药剂的选择提供参考。

Botrytis cinerea is the pathogen of many crops gray mold disease and its conidia is the major carrier for disease epidemic.In this work,the carbon utilization and conidia formation of B.cinerea were analyzed by phenomic technology.In addition,the carbon sources for conidia production of this pathogen under five different fungicides(carbendazim,propiconazole,pyrimethanil,iprodione and prochloraz)were also tested.These results showed that about 92 carbon sources could be metabolized by B.cinerea,including carbohydrates,amino acids,and others.Among them,about 35 carbon sources such as amygdalin and L-arabinose could be metabolized to produce conidia.And about 57 carbon sources(tween 80,D-arabinose,glucuronic acid,etc.)could be metabolized by B.cinerea,but could not promote the conidia formation.When treated by 1 mg/L of carbendazim and 5 mg/L of prochloraz,and at both 1 and 10 mg/L of propiconazole,the carbon sources for conidia production of B.cinerea declined greatly.While treated for 0.08 and 1 mg/L of pyrimethanil or 0.1 and 5 mg/L of iprodione,they showed no inhibition.The results mentioned above revealed the carbon sources for conidia production of B.cinerea under chemical pressures of fungicides,which give us some constructive suggestions for the fungicide selection in gray mold management.