肺腺癌中细胞分裂周期相关蛋白家族预后价值的生物信息学分析

Bioinformatics analysis of the prognostic value of the cell division cycle-associated protein family in lung adenocarcinoma

目的对肺腺癌(LUAD)中细胞分裂周期相关蛋白家族(CDCAs)的预后价值进行生物信息学分析。方法通过GEPIA、Kaplan-Meier、cBioPortal、GeneMANIA、String和TIMER2.0等数据库对肺腺癌患者CDCAs的差异表达、预后价值、临床肿瘤分期、基因改变和免疫细胞浸润结果进行分析。结果LUAD组织中CDCA1~8 mRNA水平较正常组织升高,CDCA1、2、3、4、5、7、8蛋白表达水平也较正常组织升高,但CDCA6蛋白表达水平较正常组织下降。CDCA1、3、4 mRNA与LUAD患者总生存期(OS)和无病生存期(DFS)呈正相关,CDCA2、5、8仅与OS呈正相关。CDCAs的功能主要与染色体位置、着丝粒、染色体分离调控和赤道板集合等有关。CDCAs的表达与肺腺癌中CD4^(+)T细胞、巨噬细胞、中性粒细胞、B细胞、CD8;T细胞、树突状细胞等6种免疫细胞的浸润呈显著相关。结论CDCAs可能对LUAD的发生和发展产生调控作用,CDCA1、2、3、4、5、8有望成为LUAD预后生物标志物与免疫治疗的新靶点。

Aim To analyze the prognostic value of cell division cycle-associated proteins(CDCAs)in lung adenocarcinoma(LUAD)with bioinformatics.Methods GEPIA,Kaplan-Meier,cBioPortal,String,GeneMANIA and TIMER 2.0 databases were used to analyze the differential expression,prognostic value,clinical tumor stage,gene changes and immune cell infiltration of CDCAs in patients with lung adenocarcinoma.Results The mRNA level of CDCA1-8 and the expression level of CDCA1,2,3,5,7,8 in LUAD tissues were also higher than that in normal tissues,but the expression level of CDCA6 was lower than that in normal tissues.CDCA1,3,4 mRNA was postively correlated with overall survival(OS)and disease-free survival(DFS)in patients with LUAD,while CDCA2,5,8 gene was only postively correlated with OS.The function of CDCAs is mainly related to chromosome position,centromeres,chromosome separation regulation and equatorial plate collection.The expression of CDCAs was significantly correlated with the infiltration of 6 immune cells in LUAD,including CD4^(+)T cells,macrophages,neutrophils,B cells,CD8;T cells and dendritic cells.Conclusion CDCAs may regulate the occurrence and development of LUAD,and CDCA1,2,3,4,5,8 may be prognostic biomarkers and novel targets for immunotherapy of LUAD.