5种塞内卡病毒灭活疫苗佐剂的比较研究

Comparison Study on Five Adjuvants of Seneca Virus Inactivated Vaccine

为研究比较5种塞内卡病毒疫苗佐剂,分别用4种自主研发佐剂和进口ISA206佐剂配制塞内卡病毒灭活疫苗,检测疫苗的理化性质、安全性及免疫效力,并对检测结果进行对比分析。结果显示,进口ISA206佐剂疫苗黏度为43.02 cP,自主研发佐剂疫苗黏度均在27.88 cP以下,其他理化性质无差异;5批疫苗安全性试验均未见明显异常;5批疫苗免疫动物后均可诱导机体产生中和抗体,二免后14 d抗体滴度在2^(11)以上,4批自主研发佐剂疫苗免疫组抗体滴度水平高于进口ISA206佐剂疫苗约1个滴度,攻毒保护结果均在4/5以上,2批自主研发佐剂疫苗免疫组保护率可达5/5。结果表明,用4种自主研发佐剂制备的塞内卡病毒灭活疫苗质量不低于用进口ISA206佐剂制备的塞内卡病毒灭活疫苗。

In order to study and compare five adjuvants of Seneca virus vaccine,four independently developed adjuvants and imported ISA206 adjuvant were used to prepare Seneca virus inactivated vaccine,the physical and chemical properties,safety and immune efficacy of the vaccine were detected.The results showed that the viscosity of ISA206 adjuvant vaccine was 43.02 cP,and the viscosity of independently developed adjuvant vaccines was below 27.88 cP,and there was no difference in other physical and chemical properties.No obvious abnormality was found in the safety test of five batches of vaccines.The body was all induced to produce neutralizing antibody after five batches of vaccines immunized to animals,and the titer was all over 2^(11)fourteen days after second immunization.The antibody concentration of four batches of independently developed adjuvant vaccines was higher than that of ISA206 adjuvant vaccine about one degree,tapping poison protection results was more than 4/5,in which two batches of independently developed adjuvant vaccines could reach 5/5.In conclusion,the quality of the inactivated Seneca virus vaccine prepared with four independently developed adjuvants was not lower than that of the inactivated Seneca virus vaccine prepared with imported ISA206 adjuvant.