光照强度对中缅树鼩行为、学习记忆和氧化应激的影响

Effects of light intensity on behavior,learning memory and oxidative stress of Tupaia belangeri

为探讨不同光照强度对中缅树鼩行为变化、学习记忆能力和氧化应激的影响,分别于光照628、1256和2512 lx条件下测定体重、摄食量、Y型迷宫正确反应率和行为变化,并测定丙二醛(Malondialdehyde,MDA)浓度、超氧化物歧化酶(Superoxide dismutase,SOD)、乙酰胆碱酯酶(Acetylcholinesterase,AChE)和一氧化氮合酶(Nitric oxide synthase,NOS)浓度,以及SOD、AChE和NOS的活性。结果表明,第28天时3组之间体重差异显著,摄食量的变化趋势和体重变化相似。强光照明显降低中缅树鼩的取食行为,增加休息行为。第28天时,不同光照强度组之间识别正确率差异极显著。不同光照强度对MDA、SOD浓度和SOD活性影响差异极显著,对NOS和AChE活性影响极显著。结果说明,强光照可以降低中缅树鼩的体重和取食行为,增加休息行为,可诱发中缅树鼩的认知障碍和脑组织内不同程度的氧化损伤。

To investigate the effects of different light intensities on the behavior changes,learning memory ability and oxidative stress in Tupaia belangeri,body mass,food intake,behavior and correct response rate in Y-maze,concentrations of malondialdehyde(MDA),superoxide dismutase(SOD),acetylcholinesterase(AChE)and nitric oxide synthase(NOS),as well as activities of SOD,AChE and NOS were measured in T.belangeri,which were continuously exposed to 628,1256 and 2512lx.The results showed that body mass had significant differences among three groups,changes of food intake were similar to body mass in T.belangeri.Higher light intensity re-duced feeding behavior and increased resting behavior significantly.On day 28,there was a significant difference in recognition accuracy among different light intensity groups.Different light intensity had significant effects on the concentrations of MDA,concentration and ac-tivity of SOD,and the activities of NOS and AChE.All of the results indicated that higher light intensity reduced body mass and feeding behavior,increased rest behavior,and induced cognitive impairment and caused different degrees of oxidative damage in T.belangeri.