浸润性乳腺癌PELP1/MNAR表达与雌激素受体、人表皮生长因子受体2、Ki-67表达及PIK3CA基因突变的相关性分析

Analysis of the correlation of PELP1/MNAR expression with expressions of estrogen receptor,human epidermal growth factor receptor 2,Ki-67 and PIK3CA gene mutation in invasive breast cancer

目的探讨浸润性乳腺癌PELP1/MNAR表达与雌激素受体(ER)、Ki-67和人表皮生长因子受体2(HER2)表达及与PIK3CA基因突变的相关性。方法收集山西省大同市第五人民医院2008年1月至2018年12月80例原发浸润性乳腺癌患者石蜡包埋组织标本,应用免疫组织化学EliVision二步法检测PELP1/MNAR蛋白表达,采用聚合酶链反应(PCR)-Sanger测序检测PIK3CA基因突变情况。比较ER、Ki-67、HER2不同表达状态及PIK3CA基因是否突变患者间PELP1/MNAR表达情况,分析各指标与PELP1/MNAR表达的相关性。结果ER阳性[86.1%(31/36)比59.1%(26/44)]、Ki-67高表达[100.0%(13/13)比65.7%(44/67)]、HER2阳性[81.0%(34/42)比60.5%(23/38)]患者PELP1/MNAR蛋白高表达率较高,差异均有统计学意义(均P<0.05)。PIK3CA突变型和野生型患者PELP1/MNAR蛋白高表达率差异无统计学意义[60.0%(12/20)比75.0%(45/60),P=0.199]。PELP1/MNAR的表达与ER表达水平呈负相关(r=-0.195,P<0.05),与Ki-67表达水平呈正相关(r=0.198,P<0.05),与HER2表达水平呈正相关(r=0.225,P<0.05),与淋巴结转移呈负相关(r=-0.269,P<0.05)。结论浸润性乳腺癌PELP1/MNAR的表达与ER表达水平负相关,与Ki-67、HER2表达水平正相关。PELP1/MNAR表达与PIK3CA基因突变无相关性,二者可能在乳腺癌PI3K-AKT-mTOR调节途径中各自发挥作用。

Objective To investigate the correlation of PELP1/MNAR expression with expressions of estrogen receptor(ER),Ki-67,human epidermal growth factor receptor 2(HER2)and PIK3CA gene mutation.Methods A total of 80 paraffin-embedded tissue specimens of primary invasive breast cancer patients in the Fifth People's Hospital of Datong in Shanxi Province from January 2008 to December 2018 were collected.The expression of PELP1/MNAR was examined by immunohistochemistry EliVision tow-step method.The polymerase chain reaction(PCR)-Sanger sequencing method was used to detect the mutation of PIK3CA gene.The expressions of PELP1/MNAR among patients with different expression status of ER,Ki-67,HER2 and with or without PIK3CA gene mutation were compared,and the correlations between each index and the expression of PELP1/MNAR were analyzed.Results The high expression rates of PELP1/MNAR protein in patients with ER-positive[86.1%(31/36)vs.59.1%(26/44)],Ki-67 high expression[100.0%(13/13)vs.65.7%(44/67)],HER2-positive[81.0%(34/42)vs.60.5%(23/38)]were high,and the differences were statistically significant(all P<0.05).There was no significant difference in the high expression rate of PELP1/MNAR protein between patients with mutant and wild-type PIK3CA[60.0%(12/20)vs.75.0%(45/60),P=0.199].The expression of PELP1/MNAR was negatively correlated with the expression level of ER(r=-0.195,P<0.05),positively correlated with the expression level of Ki-67(r=0.198,P<0.05),positively correlated with the expression level of HER2(r=0.225,P<0.05),and negatively correlated with lymph node metastasis(r=-0.269,P<0.05).Conclusions The expression of PELP1/MNAR in invasive breast cancer is negatively correlated with the expression level of ER,and positively correlated with the expression level of Ki-67 and HER2.There is no correlation between PELP1/MNAR expression and PIK3CA gene mutation,and the two may play their own role in the PI3K-AKT-mTOR regulatory pathway of breast cancer.