miR-143/145启动子区rs17723799多态性与宫颈癌易感性的相关性研究

Correlation of miR-143/145 promoter region rs17723799 polymorphism with susceptibility to cervical cancer

目的:探讨miR-143/145启动子区rs17723799多态性与宫颈癌的关系。方法:选取242例宫颈癌患者及249例健康志愿者作为对照,采用SnaPshot和Sanger测序法对rs17723799进行多态性检测,对临床数据进行分层分析,qRT-PCR检测宫颈癌组织中miR-143/145相对表达。结果:rs1723799 TC和TT基因型降低了宫颈癌发病风险(P<0.05),分层分析显示,携带rs1723799 TC/TT基因型的患者淋巴结转移风险降低(P=0.028),此外,携带rs1723799 TC基因型的患者与携带rs1723799 CC/TC基因型的患者相比,miR-143/145表达水平更高(P=0.011,P=0.014)。结论:miR-143/145启动子区rs1723799多态性可能与宫颈癌遗传易感性相关。

Objective:To investigate correlation of rs17723799 polymorphism in promoter region of miR-143/145 with cervical cancer.Methods:242 cervical cancer patients and 249 healthy controls were enrolled. rs17723799 polymorphism was genotyped by SnaPshot and Sanger sequencing. Relative expression of miR-143/145 in cervical cancer tissues was examined by qRT-PCR. Clinical data were also analyzed.Results:rs1723799 TC and TT genotypes had an decreasing risk of cervical cancer(P<0.05). Stratified analysis showed that patients carrying rs1723799 TC/TT genotypes had an decreasing risk of lymph node metastasis(P=0.028). Moreover,patients carrying rs1723799 TC genotype had a higher level of miR-143/145 compared with those carrying rs1723799 CC/TC genotype(P=0.011,P=0.014).Conclusion:rs1723799 polymorphism in miR-143/145 promoter region may be associated with genetic susceptibility to cervical cancer.