摘要
Small RNAs(sRNAs)are essential regulatory molecules,and there are three major sRNA classes in plants:microRNAs(miRNAs),phased small interfering RNAs(phased siRNAs or phasiRNAs),and heterochromatic siRNAs(hc-siRNAs).Excluding miRNAs,the other two classes are not well annotated or available in public databases for most sequenced plant genomes.We performed a comprehensive sRNA annotation of 143 plant species that have fully sequenced genomes and next-generation sequencing sRNA data publicly available.The results are available via an online repository called sRNAanno(www.plantsRNAs.org).Compared with other public plant sRNA databases,we obtained was much more miRNA annotations,which are more complete and reliable because of the consistent and highly stringent criteria used in our miRNA annotations.sRNAanno also provides free access to genomic information for>22,721 PHAS loci and>22 million hc-siRNA loci annotated from these 143 plant species.Both miRNA and PHAS loci can be easily browsed to view their main features,and a collection of archetypal trans-acting siRNA 3(TAS3)genes were annotated separately for quick access.To facilitate the ease of sRNA annotation,sRNAanno provides free service for sRNA annotations to the community.In summary,the sRNAanno database is a great resource to facilitate genomic and genetic research on plant small RNAs.
基金
the National Key Research and Developmental Program of China(2018YFD1000104)
the National Natural Science Foundation of China(#31872063)
the Special Support Program of Guangdong Province(2019TX05N193)
the Guangzhou Science and Technology Key Project(201804020063)
the Innovation Team Project of the Department of Education of Guangdong Province(2016KCXTD011)
the Key Areas of Science and Technology Planning Project of Guangdong Province(2018B020202011)
the Guangdong Innovative and Entrepreneurial Research Team Program(2016ZT06S172)。
