葛根素对U14宫颈癌小鼠血液流变学、脾淋巴细胞增殖及细胞毒性影响

Effects of Puerarin on Hemorheology, Splenic Lymphocyte Proliferation and Cytotoxicity in U14 Cervical Cancer Mice

目的:研究葛根素治疗对U14宫颈癌小鼠血液流变学、脾淋巴细胞增殖活性及对宫颈癌细胞毒性的影响。方法:45只雌性昆明小鼠随机分为对照组、模型组和葛根素组。模型组和葛根素组小鼠通过腋下注射U14小鼠宫颈癌细胞建立U14宫颈癌移植瘤小鼠,并且葛根素小鼠通过葛根素灌胃进行治疗,对照组和模型组小鼠给予等量生理盐水。比较各组小鼠血流变学、脾淋巴细胞增殖活性及对宫颈癌细胞毒性。结果:经葛根素治疗的葛根素组宫颈癌小鼠肿瘤重量显著低于模型组小鼠(P<0.05),葛根素治疗宫颈癌小鼠的抑瘤率是(42.91±12.91)%。宫颈癌小鼠低切/高切全血粘度、血浆粘度值以及血细胞比容均显著升高(P<0.05),而葛根素治疗可显著降低宫颈癌小鼠低切/高切全血粘度、血浆粘度值以及血细胞比容(P<0.05)。宫颈癌小鼠脾脏重量、脾脏指数和脾淋巴细胞体外增殖能力均显著下降(P<0.05),而葛根素治疗可显著提高宫颈癌小鼠脾脏重量、脾脏指数和脾淋巴细胞体外增殖能力(P<0.05)。此外,经葛根素治疗的宫颈癌小鼠脾淋巴细胞对U14宫颈癌细胞细胞毒性显著高于模型组宫颈癌小鼠(P<0.05)。结论:葛根素治疗可降低U14宫颈癌小鼠血液粘度、改善血流变性质,并且可以提高脾淋巴细胞的增殖活性和对宫颈癌细胞的杀伤力。

Objective:To study the effects of puerarin treatment on hemorheology,splenic lymphocyte proliferation activity and the toxicity of cervical cancer cells in U14 cervical cancer mice.Methods:45 female Kunming mice were randomly divided into control group,model group and puerarin group.Mice in the model group and puerarin group were injected into the armpits of U14 mouse cervical cancer cells to establish U14 cervical cancer transplantation mice,and puerarin mice were treated by gavage of puerarin.The control group and model group mice were given the same amount Normal saline.Compare the blood rheology,splenic lymphocyte proliferation activity and toxicity to cervical cancer cells in each group of mice.Results:The tumor weight of cervical cancer mice in the puerarin group treated with puerarin was significantly lower than that in the model group(P<0.05).The tumor inhibition rate of cervical cancer mice treated with puerarin was(42.91±12.91) %.Cervical cancer mice low-cut/high-cut whole blood viscosity,plasma viscosity value and hematocrit increased significantly(P<0.05),and puerarin treatment can significantly reduce low-cut/high-cut whole blood viscosity,Plasma viscosity and hematocrit(P<0.05).Spleen weight,spleen index and in vitro proliferation ability of spleen lymphocytes in cervical cancer mice were significantly decreased(P<0.05),while puerarin treatment can significantly increase the spleen weight,spleen index,and in vitro proliferation ability of spleen lymphocytes in cervical cancer mice(P<0.05).In addition,the spleen lymphocytes of cervical cancer mice treated with puerarin were significantly more cytotoxic to U14 cervical cancer cells than the model group cervical cancer mice(P<0.05).Conclusion:Puerarin treatment can reduce the blood viscosity of U14 cervical cancer mice,improve hemorheological properties,and can increase the proliferation activity of splenic lymphocytes and the lethality of cervical cancer cells.