不同来源山楂叶化学成分比较分析

Comparative analysis of chemical constituents in hawthorn leaves from different sources

利用UHPLC-Q-TOF/MS;结合UNIFI数据分析平台和多元统计方法对山楂(来自野生)和山里红(来自栽培)两个来源山楂叶的化学成分及差异性成分进行分析与鉴定。从山楂叶中共分析鉴定化学成分58个,包括有机酸、黄酮、三萜酸、单萜及倍半萜类成分,其中萜类成分最为丰富。同时利用主成分分析(PCA)及正交偏最小二乘判别分析(OPLS-DA)对2个来源山楂叶的差异性成分进行分析,发现24个差异成分主要为黄酮和萜类成分(包括单萜、倍半萜和三萜酸),且野生的山楂叶中黄酮(如芦丁、牡荆素-2’’-O-鼠李糖苷、异牡荆素-2’’-O-鼠李糖苷、金丝桃苷、槲皮素-3-O-β-D-吡喃葡萄糖苷)和萜类(如山楂酸、科罗索酸和熊果酸)比栽培的山里红丰富,其含量也相对较高。该研究全面反映了《中国药典》中2个法定来源山楂叶化学成分的差异,为山楂叶药材基原的选择以及合理开发利用提供了依据。

We identified and analyzed the components and chemical constituents of hawthorn leaves of Crataegus pinnatifida Bge.(wild)and C.pinnatifida Bge.var major N.E.Br(cultivated)by using ultra highperformance liquid chromatography and quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF/MS;)combined with UNIFI data analysis platform and multivariate statistics.Fifty-eight chemical compounds were identified,including organic acids,flavonoids,triterpenoic acids,monoterpenes and sesquiterpenoids;among them,terpenoid content was the most abundant.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to identify the differential components of hawthorn leaves from two sources.The results show that there are differences in the chemical compositions of the two sources,including 24 flavonoids and terpenoids(including monoterpenoids,sesquiterpenoids and triterpenoid acids).The types of flavonoids(such as rutin,vitexin-2’’-O-rhamnoside,isovitexin-2’’-O-rhamnoside,hyperoside,quercetin-3-O-β-Dglucoside)and terpenoids(crataegolic acid,corosolic acid and ursolic acid)in C.pinnatifida were more varied than those found in C.pinnatifida Bge.var major N.E.Br,and their contents were relatively higher.This study provides a comprehensively analysis of the different chemical components of hawthorn leaves from two sources listed in the Chinese Pharmacopoeia,and provides a basis for the selection of raw materials and the potential development and utilization of hawthorn leaves.