六堡茶水提物对小鼠腹泻及肠道菌群的影响

Effects of Liupao tea water extract on diarrhea and intestinal flora of mice

目的研究六堡茶水提物对番泻叶诱导的小鼠腹泻及肠道菌群的影响。方法采用六堡茶水提物对小鼠进行20 d的腹泻预防灌胃,从第16 d开始同时给予小鼠番泻叶煎剂诱导小鼠腹泻模型;实验结束时观察各组小鼠行为状态、体重、腹泻率及腹泻指数,检测盲肠内容物pH及氨态氮含量、粪便肠道菌群数量,分析空肠绒毛形态结构。结果与正常组相比,模型组小鼠体重显著下降(P<0.05)、稀便率和腹泻指数均极显著上升(P<0.01),说明番泻叶诱导小鼠腹泻模型构建成功。与模型组相比,六堡茶防治组特别是高剂量组显著降低了小鼠稀便率和腹泻指数(P<0.05),显著降低了小鼠盲肠内容物pH及氨态氮含量(P<0.05),显著增加了小鼠肠道双歧杆菌数量以及减少了肠球菌数量(P<0.05),显著提升了小鼠空肠绒毛长度与隐窝深度的比值(P<0.05)。结论六堡茶水提物对小鼠腹泻具有一定防治作用,其止泻作用可能与改善肠道内环境及修复双歧杆菌和肠球菌数量有关。

Objective To study the influence of Liupao tea water extract on diarrhea and intestinal flora of mice induced by senna leaves.Methods Mice were administrated with Liupao tea water extract to prevent diarrhea in mice for 20 d,and were simultaneously given senna leaves decoction to induce diarrhea from the 16 th day.At the end of the experiment,the behavioral status,body weight,loose stools rate and diarrhea index of mice in each group were observed;the pH and ammonia nitrogen level of cecum contents,and the number of fecal intestinal flora were detected,and the morphological structure of jejunum villi was analyzed.Results Compared with the normal group,the weight loss significantly(P<0.05),the loose stools rate and diarrhea index of mice in the model group were extremely significantly increased simultaneously(P<0.01),indicating that the diarrhea model induced by senna leaf was successfully constructed.Compared with the model group,Liupao tea control group,especially the high-dose group,could significantly improve the loose stools rate and diarrhea index of mice(P<0.05),reduce the pH value and ammonia nitrogen content of cecum contents(P<0.05),increase the number of intestinal Bifidobacterium,reduce the number of Enterococcus(P<0.05),and repair the villus height to crypt depth in jejunum of mice(P<0.05).Conclusion Liupao tea water extract has a certain prevention effect on diarrhea of mice,and its anti-diarrhoea effect may be related to the improvement of intestinal environment and the reversal of content of the Bifidobacterium and Enterococcus.