高效液相色谱法同时测定麻花中16种多环芳烃含量

Simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons in fried dough twists by high performance liquid chromatography

目的建立高效液相色谱法测定麻花中16种多环芳烃(polycyclic aromatic hydrocarbons,PAHs)含量的分析方法。方法样品经正己烷提取,提取液经弗洛里硅藻土固相萃取小柱串联PAHs专用固相萃取小柱净化,Waters PAH C;柱进行色谱分离,采用高效液相色谱法测定,外标法定量。结果苊烯在5~200 ng/mL范围内呈现良好的线性关系,相关系数(r)为0.9996,其他15种多环芳烃在1~200 ng/mL范围内呈现良好的线性关系,相关系数(r)为0.9985-0.9998。方法的检出限为0.4~2.5μg/kg。在5、10、25μg/kg 3个添加水平的平均回收率为63%~95%,相对标准偏差小于10%。结论该方法净化效果好,具有较好的准确度、灵敏度和精密度,可用于麻花中多环芳烃的测定。

Objective To establish a method for the simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons(PAHs)in fried dough twists by high performance liquid chromatography.Methods The samples were extracted by n-hexane,and purified by Florian diatomite solid phase extraction column and PAHs-specific solid phase extraction column in series.The chromatographic separation was performed on Waters PAH C;chromatographic column,determined by high performance liquid chromatography,and quantified by external standard method.Results Acenaphthylene showed good linear relationship in the range of 5-200 ng/mL,and the correlation coefficient(r)was 0.9996,other 15 kinds of polycyclic aromatic hydrocarbons showed good linear relationship in the range of 1-200 ng/mL,and the correlation coefficients(r)were 0.9985-0.9998.The limits of detection were 0.4-2.5μg/kg.The average recoveries at 3 concentration levels of 5,10,25μg/kg were in the range of 63%-95%,and the relative standard deviations were no more than 10%.Conclusion The method has good purification effect,good accuracy,sensitivity and precision,and can be used for the determination of polycyclic aromatic hydrocarbons in fried dough twists.