聚己二酸丁二醇酯-对苯二甲酸丁二醇酯/Ⅰ型胶原取向纤维促进前交叉韧带断裂后腱-骨愈合

Aligned poly(butylene adipate-co-terephthalate)/type Ⅰ collagen fibers promote tendon-bone healing after anterior cruciate ligament rupture

背景:材料表面的微/纳米结构对细胞的行为具有调控作用,肌腱组织主要由平行的胶原纤维组成,因此取向纤维结构对腱-骨愈合具有一定的促进作用。目的:探索聚己二酸丁二醇酯-对苯二甲酸丁二醇酯[poly(butylene adipate-co-terephthalate),PBAT]/Ⅰ型胶原取向纤维支架的生物相容性和成骨诱导活性。方法:采用静电纺丝技术分别制备无序PBAT、取向PBAT和取向PBAT/Ⅰ型胶原纤维支架材料,表征3种支架的静接触角与力学性能。将3种支架分别与大鼠骨髓间充质干细胞共培养,检测细胞黏附率与增殖情况,DAPI染色和Live/Dead荧光染色观察细胞在支架上的黏附和存活情况,碱性磷酸酶染色和Ⅰ型胶原免疫组化染色检测取向纤维支架的成骨诱导活性。结果与结论:①取向PBAT/Ⅰ型胶原纤维支架的静接触角低于无序PBAT、取向PBAT纤维支架(P<0.05);②两种取向支架的应变程度优于无序PBAT支架(P<0.05),取向PBAT支架的抗拉强度优于无序PBAT组(P<0.05),3组支架的弹性模量比较差异无显著性意义(P>0.05);③取向PBAT/Ⅰ型胶原纤维支架上的细胞黏附与增殖情况优于其他两组支架;④DAPI染色和Live/Dead荧光染色显示,骨髓间充质干细胞均可在3组支架上黏附和增殖,其中以取向PBAT/Ⅰ型胶原纤维支架上的细胞存活数量最多;⑤碱性磷酸酶染色和Ⅰ型胶原免疫组化染色显示,取向PBAT/Ⅰ型胶原纤维支架的成骨诱导活性强于取向PBAT纤维支架;⑥结果表明,PBAT/Ⅰ型胶原取向纤维支架具有良好的生物相容性和成骨诱导活性,能有效促进细胞增殖、黏附。

BACKGROUND:The mic ro/nano structure on the surfa ce of the material has a regulatory effect on the behavior of the cells.The tendon tissue is mainly composed of parallel collagen fibers,so the aligned fiber structure has a certain promotion effect on tendon-bone healing.OBJECTIVE:To explore the biocompatibility and osteoinductive activity of poly(butylene adipate-co-te rephthalate)(PBAT)/type Ⅰ collagen aligned fiber scaffolds.METHODS:Electros pinning technology was used to prepare random PBAT,aligned PBAT,and aligned PBAT/type Ⅰ collagen fiber scaffold mate rials.The static conta ct angle and mechanical properties of the three stents were chara cterized.The three scaffolds were co-cultured with rat bone marrow mesenchymal stem cells to detect cell adhesion rate and proliferation.DAPI staining and Live/Dead fluorescent staining were used to observe cell adhesion and survival on the scaffold.Alkaline phosphatase staining and type Ⅰ collagen immunohistochemical staining were used to detect the osteoinductive activity of oriented fiber scaffolds.RESULTS AND CONCLUSION:(1) The static conta ct angle of oriented PBAT/type Ⅰ collagen fiber scaffold was lower than that of random PBAT and aligned PBAT fiber scaffolds(P <0.05).(2) The strain degree of the two aligned scaffolds was better than that of the aligned PBAT scaffold(P <0.05),and the tensile strength of the aligned PBAT scaffold was better than that of the random PBAT group(P <0.05).There was no significant difference in the elastic modulus of the three gro ups of scaffolds(P> 0.05).(3) The cell adhesion and proliferation on the aligned PBAT/type I collagen fiber scaffold were better than those of the other two scaffolds.(4) DAPI staining and Live/Dead fluorescent staining showed that bone marrow mesenchymal stem cells could adhere and prolife rate on the three gro ups of scaffolds,and the number of cells on the aligned PBAT/type Ⅰ collagen fiber scaffold was the largest.(5) Alkaline phosphatase staining and type Ⅰcollagen immunohistochemical staining showed that the osteoinductive activity of the aligned PBAT/type I collagen fiber scaffold was stronger than that of the aligned PBAT fiber scaffold.(6) The results show that the PBAT/type Ⅰ collagen aligned fiber scaffold has good biocompatibility and osteoinductive activity and can effectively promote cell prolife ration and adhesion.