高效液相色谱法同步测定甜叶菊提取物中7种绿原酸

Simultaneous determination of 7 chlorogenic acids in Stevia extract by high performance liquid chromatography

为建立高效液相色谱法测定甜叶菊提取物中绿原酸、新绿原酸、隐绿原酸、洋蓟素、异绿原酸B、异绿原酸A、异绿原酸C 7种绿原酸及其类似物的分析方法,本实验以超声提取法对样品进行前处理,以甲酸溶液-乙腈作为流动相,采用梯度淋洗和C_(18)色谱柱(250 mm×4.6 mm,5μm)进行各化合物分离。结果显示:7种绿原酸及其类似物的检出限为0.47~1.18 mg/kg,相关系数r均大于0.9990,加标回收率为95.10%~102.63%,相对标准偏差(n=3)为1.7%~4.1%。将本方法应用于甜叶菊提取物中的绿原酸及其类似物的含量分析,实验结果满意。

An analytical method for 7 chlorogenic acids of chlorogenic acid,5-caffeoylquinic acid,4-dicaffeoylquinic acid,1,5-dicaffeoylquinic acid,isochlorogenic acid A,isochlorogenic acid B and isochlorogenic acid C in Stevia extract was established by high performance liquid chromatography.Chlorogenic acids were released by ultrasonic pretreatment.The analyses were performed on a C_(18) column with the gradient elution of acetonitrile-formic acid water at a flow rate of 1.0 mL/min.The results showed that the method was suitable for the determination of 7 chlorogenic acids.The limits of detection were 0.47~1.18 mg/kg.The correlation coefficients were greater than 0.9990.The recoveries were observed in the range of 95.1%~102.6%.This method was applied to determine chlorogenic acids in Stevia extract products,and the results were satisfactory.