摘要
本实验通过高效液相色谱法对饲料中6种游离氨基酸含量进行测定。采用0.1 mol/L盐酸溶液提取样品中的6种游离氨基酸,与1%2,4-二硝基氟苯(DNFB)溶液衍生后经高效液相色谱仪测定。结果表明:饲料中6种游离氨基酸平均回收率为97.52%,检出限≥0.006%(信噪比S/N=3),定量限≥0.021%(信噪比S/N=10),重复性以变异系数计≥2.55%。综上,该方法适合饲料中6种游离氨基酸的测定。
High performance liquid chromatography(HPLC)method for determination of six free amino acids in feed.Six free amino acids were extracted by 0.1mol/L hydrochloric acid solution,derivatived by 1%2,4-Dinitrofluorobenzene,and determined with HPLC.The results showed that the mean recoveries of feed was 97.52%.The limit of detection(LOD)≥0.006%(S/N=3)and the limit of quantitation(LOQ)≥0.021%(S/N=10).The repeatability was calculated as the coefficient of variation≥2.55%.This method could be used for quantitive analysis of six free amino acids in feed.
作者
刘成模
杨曦
莫彩娜
陈小燕
任秋香
黄智成
LIU Chengmo;YANG Xi;MO Caina;CHENG Xiaoyan;REN Qiuxiang;HUANG Zhicheng(Guangdong Evergreen Feed Industry Co.,Ltd.,Zhanjiang,Guangdong Province 524094,China;High performance liquid chromatography(HPLC)method for determination of six free amino acids in feed,Zhanjiang,Guangdong Province 524094,China)
出处
《中国饲料》
北大核心
2022年第3期99-102,共4页
China Feed
基金
广东省重点领域研发计划项目(2020B0202010001)
2020年度湛江市科技发展专项资金竞争性分配项目(2020A0612)
2019年度湛江市非资助科技攻关计划项目(2019B01007)。