150份青稞种质资源成株期耐旱性鉴定

Identification of Drought Tolerance of 150 Hulless Barley Germplasm Resources at Adult Stage

为了解青藏高原地区青稞种质材料的耐旱性,有效利用青稞自身耐旱遗传潜力,为耐旱育种亲本选配提供参考,以150份青稞种质资源为研究材料,连续2 a检测在正常灌水和干旱胁迫2个水平处理下各青稞种质资源成熟期7个与产量相关的指标,利用耐旱性综合评价(D)、综合耐旱系数(CDC)和加权耐旱系数(WDC)相结合的方法进行耐旱指标筛选及资源的耐旱性鉴定。结果显示,利用主成分分析(PCA)可将7个与产量相关的指标简化为4个公因子,并携带原有7个指标88.26%的数据信息量,其中单株产量可作为比较直观的耐旱评价指标。综合这3种分析方法,筛选出7个成株期耐旱性强的青稞种质资源。主穗穗数、单株粒质量、主穗长和单株粒数可以作为青稞成株期耐旱性简单且较为准确的评价指标。

In order to understand hulless barley’s tolerance to drought,hulless barley’s excellent genetic potrential in drought-tolerant was effectively exploited to provid references for selecting drought-tolerant parents in hulless barley breeding.Taking 150 accessions of hulless barley germplasm resources as materials,we counted seven indexes related to yield,such as main panicle length,grain number per plant,grain mass per plant,thousand grain mass,spikelet number of the main spike,plant height and effective panicle at the mature stage under normal irrigation and drought stress.The drought tolerance index was screened,and the drought tolerance of the resources was identified by combining drought tolerance evaluation(D),drought tolerance coefficient(CDC),and weighted drought tolerance coefficient(WDC).The results showed that the 7 indexes could be simplified into 4 common factors by using principal component analysis(PCA),which accounted for 88.26%of the original 7 indexes.Among them,the yield per plant could be used as a more intuitive evaluation index of drought tolerance.24,12,15,14,109,7 and 111 were identified as strong drought tolerance germplasm at the adult plant.The results showed that the spikelet number of the main spike,grain mass per plant,main spike length,and grain number per plant can be used as simple and accurate evaluation indexes for drought tolerance of hulless barley at the adult plant stage.