B细胞淋巴瘤患者TRIP13mRNA表达水平与临床特征的关系

Expression of TRIP13 mRNA and its relation with clinical features of patients with B-cell lymphoma

目的探讨B细胞淋巴瘤患者淋巴瘤组织或骨髓组织(受淋巴瘤侵犯)中甲状腺激素受体相互作用因子13(TRIP13)mRNA表达水平的变化及其与临床特征的关系。方法选取2018年9月1日至2020年11月30日在嘉兴市第二医院就诊,进行淋巴结活检或骨髓穿刺活检的患者66例,留取患者多余的新鲜淋巴结活检组织或新鲜骨髓活检组织(受淋巴瘤侵犯)冻存。采用RT-PCR法检测组织中TRIP13 mRNA表达水平。比较惰性B细胞淋巴瘤组(22例)、侵袭性B细胞淋巴瘤组(20例)、淋巴结炎组(24例)患者TIRP13 mRNA表达水平;分析TRIP13 mRNA表达水平对B细胞淋巴瘤的诊断效能;以TRIP13 mRNA表达水平诊断B细胞淋巴瘤的最佳截断值为分界点,将B细胞淋巴瘤患者分为TRIP13 mRNA低表达组(12例)及TRIP13 mRNA高表达组(30例),比较两组患者临床特征。结果淋巴结炎组患者TIRP13 mRNA表达水平低于惰性B细胞淋巴瘤组、侵袭性B细胞淋巴瘤组(均P<0.05),而惰性B细胞淋巴瘤组与侵袭性B细胞淋巴瘤组患者TIRP13 mRNA表达水平比较差异无统计学意义(P>0.05)。以B细胞淋巴瘤与淋巴结炎为状态变量作ROC曲线,AUC为0.709,最佳截断值为0.215,灵敏度为0.721,特异度为0.652(P<0.05)。与TRIP13 mRNA低表达组相比,高表达组患者IgH基因重排阳性率较高(76.7%比41.7%,P<0.05),4个疗程后获得完全缓解率较低(33.3%比66.7%,P<0.05)。结论B细胞淋巴瘤患者TRIP13 mRNA表达水平明显升高,测定其水平有助于鉴别B细胞淋巴瘤组织和淋巴结炎组织,且其对临床早期治疗反应预判有一定价值。

Objective To investigate the expression of thyroid hormone receptor interactor 13(TRIP13)mRNA and its relationship with clinical features of patients with B-cell lymphoma.Methods Sixty six patients who underwent lymph node biopsy or bone marrow aspiration biopsy in Jiaxing Second Hospital from September 2018 to November 2020 were enrolled in the study.The biopsy confirmed indolent B-cell lymphoma in 22 cases,aggressive B-cell lymphoma in 20 cases and lymphadenitis in 24 cases.The expression levels of TRIP13 mRNA in lymph node or bone marrow specimens were detected with RT-PCR and compared among three groups.The diagnostic efficacy of TRIP13 mRNA expression levels for B-cell lymphoma was analyzed.The correlation between TRIP13 mRNA expression level and clinical characteristics of patients with B-cell lymphoma was analyzed.Results The level of TIRP13 mRNA in the lymphadenitis group was lower than that in the indolent B-cell lymphoma group and aggressive B-cell lymphoma group(all P<0.05).However,there was no significant difference in TIRP13 mRNA level between the indolent B-cell lymphoma group and the aggressive B-cell lymphoma group(P>0.05).The area under ROC curve(AUC)of TIRP13 mRNA expression for differential diagnosis between B-cell lymphoma and lymphadenitis was 0.709(P<0.05),the best cut-off value was 0.215,and the sensitivity and specificity was 0.721 and 0.652,respectively.Compared with the TRIP13 mRNA low expression group,the positive rate of IgHgene rearrangement in the high expression group was higher(76.7%vs.41.7%,P<0.05),and the complete remission rate was lower after 4 courses of treatment(33.3%vs.66.7%,P<0.05).Conclusion The level of TRIP13 mRNA in patients with B-cell lymphoma is significantly increased.Determination of its level is helpful to dis-tinguish B-cell lymphoma from lymphadenitis,and it has a certain value in predicting the response to early clinical treatment in B-cell lymphoma patients.