长链非编码RNA ST7-AS1靶控miR-580-3p对卵巢癌细胞增殖、迁移及侵袭能力的影响研究

Effects of LncRNA ST7-AS1 on proliferation,migration and invasion of ovarian cancer cells through targeting miR-580-3p

目的探讨长链非编码RNA ST7-AS1对卵巢癌细胞增殖、迁移及侵袭能力的影响及与其与miR-580-3p的调控机制。方法选取2019年6月至2021年6月绍兴市人民医院30例卵巢癌患者手术切除的上皮性卵巢癌(EOC)组织及配对的癌旁正常组织。采用荧光实时定量PCR(RT-qPCR)检测EOC及癌旁正常组织ST7-AS1表达水平;小干扰RNA(siRNA)转染技术下调卵巢癌A2780细胞株中ST7-AS1的表达水平,将细胞分为si-ST7-AS1组和si-NC组。CCK-8法检测两组细胞的增殖能力,Transwell迁移及侵袭实验检测两组细胞的迁移及侵袭能力。双荧光素酶报告试验验证ST7-AS1靶控miR-580-3p。RT-qPCR检测si-ST7-AS1组和si-NC组细胞miR-580-3p表达水平。结果与癌旁正常组织相比,EOC组织中ST7-AS1表达水平升高(P<0.05)。si-ST7-AS1组卵巢癌细胞ST7-AS1表达水平明显低于si-NC组(P<0.05),即转染成功。Si-ST7-AS1组细胞的增殖、迁移及侵袭能力均较si-NC组明显减弱(均P<0.05)。ST7-AS1靶向调控miR-580-3p,卵巢癌A2780细胞抑制ST7-AS1表达后miR-580-3p表达水平升高(P<0.05)。结论ST7-AS1在EOC组织中表达水平较癌旁正常组织升高,其可能通过负性调控miR-580-3p促进卵巢癌细胞的增殖、迁移及侵袭等恶性生物学行为。

Objective To investigate the effects of lncRNA ST7-AS1 on proliferation,migration and invasion of ovarian cancer cells and its regulatory mechanism with miR-580-3p.Methods Samples of tumor tissues and corresponding adjacent normal ovarian tissues surgically removed from 30 patients with epithelial ovarian cancer(EOC)in Shaoxing People's Hospital from June 2019 to June 2021 were collected.The expression of ST7-AS1 in EOC and corresponding normal tissues was detected by fluorescence real-time quantitative RT-PCR(qRT-PCR).Human ovarian cancer A2780 cells were transfected with si-ST7-AS1 or si-NC group.The cell proliferation was detected by CCK-8 assay,and the cell migration and invasion were detected by Transwell assay.Dual luciferase report assay was applied to verify ST7-AS1 targeting miR-580-3p.qRT-PCR was applied to detect the expression of miR-580-3p in si-ST7-AS1 group and si-NC group.Results The expression of ST7-AS1 in EOC tissues was significantly higher than that in adjacent normal tissues(P<0.05).The relative expression of ST7-AS1 in si-ST7-AS1 group was significantly decreased compared to si-NC group,indicating that the transfection was successful.The abilities of proliferation,migration and invasion of cells in si-ST7-AS1 group were significantly lower than that in si-NC group(P<0.05).ST7-AS1 targeted regulation of miR-580-3p.The expression of miR-580-3p was increased after inhibiting the expression of ST7-AS1 in A2780 cells(P<0.05).Conclusion The upregulation of ST7-AS1 may promote the proliferation,migration and invasion of ovarian cancer cells by negatively regulating miR-580-3p.