单质硫冲击对含硫废水中硫细菌与聚糖菌竞争关系的影响

Effects of elemental sulfur shock on the competition between sulfur bacteria and glycogen-accumulating organisms in sulfate-containing wastewater

聚糖菌的富集会与硫循环耦合反硝化除磷系统(denitrifying sulfur conversion-associated enhanced biological phosphorous removal,DS-EBPR)内功能种群微生物——硫细菌发生竞争,从而导致除磷效果波动。因此,首先对母反应器中微生物进行了长期驯化,然后通过向批次实验小反应器中投加单质硫,研究单质硫短期冲击对含硫废水中硫细菌与聚糖菌竞争关系的影响。结果表明,经过长期驯化,在母反应器中发现了硫细菌与聚糖菌共存的现象;而短期冲击实验结果表明,在单个周期反应过程中,虽然单质硫的投加对微生物内源物质(聚羟基脂肪酸酯、糖原)转化量、氮磷去除效果影响不大,但其可以提高硫细菌的活性,增加硫转化量,使得硫细菌在与聚糖菌的竞争中取得优势地位。

Glycogen-accumulating organisms(GAOs)can compete with the functional microorganism of denitrifying sulfur conversion-associated enhanced biological phosphorous removal(DS-EBPR)system,i.e.sulfur bacteria,and lead to the fluctuations of phosphorus removal performance.Therefore,this study firstly cultivated the sulfur bacteria in the parent reactor during the long-term operation,and then conducted the experiments of the short-term shock effects of adding elemental sulfur on competition between sulfur bacteria and GAOs in batch tests.The results showed that sulfur bacteria and GAOs could coexist in one ecosystem during the long-term operation.At the same time,the short-term batch tests indicated that although adding elemental sulfur insignificantly affected the production of endogenous substances(such as PHA,glycogen),while it could improve the activity of sulfur bacteria and increase sulfur conversion,subsequently lead to the dominance of sulfur bacteria during the competition with GAOs.