临床无菌部位分离的纹带棒状杆菌对常用消毒剂的抗性研究

Study on the resistance of Corynebacterium striatum isolated from clinically sterile sites to common disinfectants

目的考察临床分离的不同基因型的纹带棒状杆菌对5种医院常用消毒剂的抗性特点,为科学消毒和预防纹带棒状杆菌引起的医院感染提供依据。方法对36株不同标本来源的纹带棒状杆菌,进行MLST分子分型。配制不同稀释倍数的消毒液,分别测定其最低抑菌浓度和最小杀菌浓度,分析不同基因型别的纹带棒状杆菌对5种消毒剂的抗性特点。结果MLST分型结果显示,36株纹带棒状杆菌共分为9个基因型别。各型别纹带棒状杆菌对5种消毒剂抗性水平基本一致,均具有很高的敏感性;但是乙醇和含氯消毒剂受浓度影响较大,随着稀释倍数的增加,杀菌效果显著减弱,甚至消失。结论纹带棒状杆菌对常用的5种消毒剂均无抗性,但需要注意乙醇和含氯消毒剂在使用过程中的浓度变化问题,使用浓度达标的消毒剂可以有效杀灭纹带棒状杆菌,预防和降低纹带棒状杆菌相关的医院感染的发生和水平传播。

Objective To investigate the resistance characteristics of clinically isolated Corynebacterium striatum with different genotypes to five commonly used disinfectants in hospitals,and provide a basis for scientific disinfection and the prevention and control of nosocomial infections caused by Corynebacterium striatum.Methods 36 strains of Corynebacterium striatum from different specimen sources were subjected to MLST molecular typing.Disinfectant solutions with different dilution multiples were prepared to determine the minimum inhibitory concentration and minimum bactericidal concentration,respectively,and the resistance characteristics of Corynebacterium striatum with different genotypes to five disinfectants were analyzed.Results MLST typing results showed that 36 strains of Corynebacterium striatum were divided into 9 genotypes.The resistance levels of all genotypes of Corynebacterium striatum to the five disinfectants were basically the same,and all of them had high sensitivity.However,ethanol and chlorine-containing disinfectants were greatly affected by concentration.With the increase of dilution ratio,the bactericidal effect was significantly weakened or even disappeared.Conclusion Corynebacterium striatum has no resistance to the five commonly used disinfectants.However,attention should be paid to the concentration change of ethanol and chlorine-containing disinfectants during use.The use of disinfectants with qualified concentration can effectively kill Corynebacterium striatum,and prevent and reduce the occurrence and horizontal transmission of Corynebacterium striatum related nosocomial infection.