水稻精米总硒含量全基因组关联分析

Genome-Wide Association Study of Total Selenium Content in Polished Rice

硒是人体必需的微量元素,我国是缺硒大国,同时又是世界上最大的稻米生产国和消费国,提高稻米中的硒含量能够有效解决居民饮食中缺硒的现状。文章检测了203份水稻栽培品种组成的核心种质精米的总硒含量,并进行了简单的统计分析和关联分析,结果显示,核心种质精米的总硒含量符合正态分布,最低值为0.04 mg/kg,最高值为0.13 mg/kg,存在较大的变异;在籼粳亚群之间,总硒含量没有显著的差异。通过全基因组关联分析,一共筛选到9个调控精米总硒含量的位点,其中位于第4号染色体上的qSe4-1、第5号染色体上的qSe5-2和第6号染色体上的qSe6与前人报道的调控水稻籽粒硒含量的位点有较好的重合,剩余6个位点未见报道。该结果为选育和开发富硒大米奠定了理论基础。

Selenium is an essential trace element for the human body and China is the major selenium-deficient country and also the largest rice producing and consuming country,increasing the selenium content in rice can effectively overcome the selenium deficiency problem in residential diet.In this study,the total selenium content of rice core collections comprised of 203 rice cultivars was measured as well as statistical analysis and correlation analysis were performed.The results showed that the total selenium content in polished rice of the 203 core collections was in accordance with the normal distribution and a large variation was observed with the lowest and highest selenium content at 0.04 mg/kg and 0.13 mg/kg respectively,no significant difference was detected between indica and japonica subpopulations.A total of 9 QTLs that regulate the total selenium content of polished rice were detected through genome-wide association analysis,among which,qSe4-1,qSe5-2 and qSe6 corresponded with the QTLs that regulate the selenium content in rice grains were reported previously,and the remaining 6 QTLs have not been reported.This result laid a theoretical foundation for the breeding and development of selenium-enriched rice.