柑橘大实蝇低覆盖度基因组及其分析

The low-coverage genome of Chinese citrus fruit fly Bactrocera minax and its analysis

为探究柑橘大实蝇Bactrocera minax的全基因组信息,以海关口岸截获的柑橘大实蝇样本为材料,提取DNA后构建350 bp短片段文库,采用Illumina二代测序平台开展全基因组测序,进行基因组组装、完整性评估和注释分析。结果表明,柑橘大实蝇的基因组大小为368.14 Mb,重复序列占比为16.27%,杂合率为0.79%,属于中等杂合度基因组。基因组组装得到43124条contigs,contig N50长度为94994 bp。BUSCO评估显示,组装的基因组可完整覆盖98.80%昆虫保守的单拷贝直系同源基因,表明该组装的完整性很高,可以满足后续分析。通过EVM注释流程整合了从头预测、同源预测和基于转录组预测等不同方法的注释结果,共预测到35655个蛋白编码基因,其中24343个基因有功能注释。

In order to obtain the whole genome information of Chinese citrus fruit fly Bactrocera minax,we chose B.minax specimen intercepted at customs port for genome sequencing.The purified genomic DNA was used to construct a 350 bp fragment library for whole genome sequencing using Illumina next-generation sequencing platform,followed by genome assembly,completeness assessment and annotation analysis.The assembled genome size was 368.14 Mb,and genome repetitive sequences accounted for 16.27%.The heterozygosity rate was 0.79%,showing a medium heterozygosity of this insect pest.The number of assembled contigs was 43124,and the length of contig N50 was 94994 bp.Moreover,the completeness of the assembled genome was 98.80%as estimated by BUSCO analysis,which suggested that the gene space was high enough for further analysis.The annotation results of de novo prediction,homology prediction and transcriptome-based prediction were combined through the EVM pipeline,yielding a total of 35655 genes in the official gene sets,and 24343 predicted genes were assigned functions successfully.