载姜黄素靶向脂质体对心肌梗死的治疗研究

Study of curcumin-loaded targeted liposomes in the treatment of myocardial infarction

目的构建抗cTnI抗体修饰的载有姜黄素的心肌梗死(MI)靶向脂质体(cT-Cur-LIP),旨在提高MI的治疗效果。方法采用薄膜分散法制备cT-Cur-LIP;运用马尔文激光粒度分析仪进行制剂表征;体外实验验证cT-Cur-LIP的摄取,体内实验验证cT-Cur-LIP对心肌梗死的治疗效果。结果制得的cT-Cur-LIP表面规整,呈椭圆形。体外结果显示,cT-Cur-LIP的摄取高于Cur和Cur-LIP,差异有统计学意义(P<0.05)。体内结果证明,cT-Cur-LIP组心肌细胞的纤维排列均匀,胞浆横纹清晰,但局部横纹消失。结论cT-Cur-LIP纳米靶向脂质体可以有效靶向缺氧心肌,为心肌梗死的治疗提供新的思路。

Objective To construction of curcumin-loaded myocardial infarction targeted liposomes(cT-Cur-LIP)modified with anti-cTnI antibody to improve the therapeutic effect of myocardial infarction.Methods The film dispersion method was used to prepare cT-Cur-LIP;the Malvern laser particle size analyzer was used for preparation characterization;the in vitro experiment verified the uptake of cT-Cur-LIP,and the in vivo experiment verified the therapeutic effect of cT-Cur-LIP on myocardial infarction.Results The surface of the prepared cT-Cur-LIP was round and oval.In vitro results showed that the uptake of cT-Cur-LIP was higher than that of Cur and Cur-LIP,and the difference was statistically significant(P<0.05).In vivo results proved that in the cT-Cur-LIP group,the fibers of cardiomyocytes were arranged uniformly,and the horizontal cytoplasmic stripes were clear,but the local horizontal stripes disappeared.Conclusion cT-Cur-LIP nano-targeted liposomes can effectively target hypoxic myocardium and provide new ideas for the treatment of myocardial infarction.