基于LC-Q/TOF-MS技术分析特基拉芽胞杆菌发酵产物的功能成分

Analysis of the Functional Components of Fermentation Products of Bacillus Tequilensis by LC-Q/TOF-MS

在微生物发酵床领域,特基拉芽胞杆菌Bacillus tequilensis有良好的应用前景,分析该菌代谢产生的功能物质,为功能菌株的开发和利用提供理论基础。以1株分离自养猪发酵床的特基拉芽胞杆菌Bacillus tequilensisFJAT-49377为试验材料,运用LC-Q/TOF-MS技术检测其发酵产物,质谱数据经分子特征提取后,与Metlin数据库进行比对,从而获得代谢产物成分信息。结果表明:从特基拉芽胞杆菌Bacillus tequilensisFJAT-49377测定代谢产物24种,包括了脂肽类、鞘脂类、脂肪酸胺、抗生素和三肽等物质种类。其中,高匹配成分(匹配得分≥90分)有16种,包括二氢神经鞘氨醇、植物鞘氨醇、表面活性素、乌达霉素B等;在高匹配成分中,相对含量≥0.5%的成分有4种,分别为2,6二氨基7羟基壬二酸(匹配得分96.32分,相对含量1.01%)、3氧代5S氨基己酸(匹配得分98.90分,相对含量0.88%)、表面活性素(匹配得分90.47分,相对含量0.86%)和胍基前克拉维胺(匹配得分91.69分,相对含量0.61%)。

Bacillus tequilensis had good application prospect in the field of microbial fermentation bed.The analysis of the functional materials produced by the metabolism of this strain would provide theoretical basis for the development and utilization of functional strains.One strain of Bacillus tequilensis FJAT-49377 which was isolated from the fermentation bed for pig raising was used as the experimental material to detect the fermentation products by LC-Q/TOF-MS technology.After the extraction of molecular feature,the mass spectrometry data were compared with the Metlin database to obtain the component information of the metabolites.The results showed that 24 metabolites were determined from Bacillus tequilensis FJAT-49377,including lipopeptides,sphingolipids,fatty acid amines,antibiotics and tripeptides,among which there were 16 highly matched components(matching score≥90),including dihydroneurosphine,phytosphingosine,surfactin,Udamycin B,etc.Among the highly matched components,there were 4 components with the relative content≥0.5%,which were 2,6-diamino-7-hydroxy-nonadiic acid(matching score of 96.32 points,relative content of 1.01%),3-oxo-5S-amino-hexanoic acid(matching score of 98.90 points,relative content of 0.88%),surfactin(matching score of 90.47 points,relative content of 0.86%),and guanidine-precursor clavulanate(matching score of 91.69 points,relative content of 0.61%).