白香丹胶囊对大鼠卵巢摘除所致的经前期焦虑症的改善作用

Effect of Baixiangdan capsule on premenstrual anxiety caused by ovariectomy in rats

目的通过系列行为学测试,比较中药白香丹胶囊(BXD)、5-羟色胺3受体(5-HT3R)特异性激动剂及其拮抗剂、大麻素受体1(CB1R)特异性激动剂及其拮抗剂对经前期烦躁障碍(PMDD)焦虑症大鼠的干预效应。方法大鼠经卵巢摘除、激素诱导动情周期和居住入侵3种复合因子构建PMDD焦虑症动物模型。后续实验分两批进行:第1批大鼠分为对照组、模型组、5-HT3R激动剂(1-phenylbiguanide,PBG)组、CB1R激动剂(WIN55212-2,WIN)组、PBG+WIN组、BXD组;第2批大鼠分为对照组、模型组、5-HT3R拮抗剂(Granisetron,GRA)组、CB1R拮抗剂(Rimonabant,RIM)组、GRA+RIM组、BXD组。药物干预结束后,利用旷场行为测试(OFT)和高架十字迷宫行为测试(EPM)评价各组大鼠焦虑样行为。结果相较于对照组,模型组大鼠的居住入侵(RIT)混合攻击行为得分显著升高(P<0.01)、OFT中央区进入次数及停留时间显著降低(P<0.05)、EPM开放臂进入次数及停留时间百分比显著降低(P<0.05);相较于模型组,PBG组、RIM组、BXD组的RIT混合攻击行为得分显著降低(P<0.01)、OFT中央区进入次数及停留时间显著升高(P<0.05)、EPM开放臂进入次数及停留时间百分比显著升高(P<0.05)。结论中药BXD、5-HT3R特异性激动剂1-phenylbiguanide、CB1R特异性拮抗剂Rimonabant可有效纠正PMDD焦虑症模型大鼠的焦虑样行为。

Objective To compare the intervention effects of the Baixiangdan capsule(BXD),the 5-hydroxytryptamine 3 receptor(5-HT3 R)-specific agonist and its antagonist,and the cannabinoid receptor 1(CB1 R)-specific agonist and its antagonist on premenstrual dysphoric disorder(PMDD)rats.Methods An animal model of PMDD anxiety was established using three compound factors:ovariectomy,hormone-induced estrous cycle and residential invasion.Follow-up experiments were carried out in two batches.The first batch of rats was divided into a blank group,a model group,a 5-HT3 R agonist(1-phenylbiguanide,PBG)group,a CB1 R agonist(WIN55212-2,WIN)group,a PBG+WIN group and a BXD group.The second batch of rats was divided into a blank group,a model group,a 5-HT3 R antagonist(Granisetron,GRA)group,a CB1 R antagonist(Rimonabant,RIM)group,a GRA+RIM group and a BXD group.After drug intervention,the anxiety-like behavior of rats in each group was evaluated using the open-field test(OFT)and elevated plus maze(EPM).Results Compared with the control group,the mixed aggressive behavior score of resident-intruder test(RIT)in the model group was significantly higher(P<0.01),and the number of entries into the central area of the OFT and the percentage of staying in the EPM open arms were significantly lower(P<0.05).Compared with the model group,the RIT mixed aggression scores of the PBG,RIM and BXD groups were significantly lower(P<0.01),the number of entries and residence time in the central area of the OFT were significantly higher(P<0.05),and the number of entries and residence time percentage of the EPM open arm were significantly higher(P<0.05).Conclusions BXD,5-HT3 R agonist PBG and CB1 R antagonist RIM can effectively correct the anxiety-like behavior of PMDD model rats.