弥漫性大B细胞淋巴瘤中miR-34a、FOXP1表达的相关性及临床意义

Correlation and clinical significance of miR-34a and FOXP1 expression in diffuse large B-cell lymphoma

目的研究弥漫性大B细胞淋巴瘤(DLBCL)中微小RNA(miR)-34a、叉头框蛋白P1(FOXP1)表达的相关性及临床意义。方法选择2018年2月至2019年7月期间经达州市中心医院病理确诊的DLBCL组织79例和反应性增生淋巴结组织60例,采用荧光定量PCR检测miR-34a的表达水平、western blot检测FOXP1的表达水平,分析miR-34a与FOXP1的相关性。随访并分析不同miR-34a、FOXP1表达的DLBCL患者无进展生存情况的差异。结果DLBCL组织中miR-34a的表达水平低于反应性增生淋巴结组织、FOXP1的表达水平高于反应性增生淋巴结组织,差异有统计学意义(P<0.05);DLBCL组织中miR-34a与FOXP1具有负相关关系(r=-0.528,P<0.05);与临床分期Ⅰ~Ⅱ期、LDH正常、IPI评分0~1分、GCB亚型DLBCL组织比较,临床分期Ⅲ~Ⅳ期、LDH升高、IPI评分≥2分、Nom-GCB亚型DLBCL组织中miR-34a的表达水平明显降低、FOXP1的表达水平明显升高差异有统计学意义(P<0.05);miR-34a表达水平≥中位数的DLBCL患者无进展生存时间较miR-34a表达水平<中位数的DLB CL患者延长,FOXP1表达水平≥中位数的DLB CL患者无进展生存时间较FOXP1表达水平<中位数的DLBCL患者缩短。结论miR-34a低表达、FOXP1高表达与DLBCL的病理进展及预后恶化有关。

Objective To study the correlation and clinical significance of microRNA(miR)⁃34a and forkhead box protein P1(FOXP1)expression in diffuse large B⁃cell lymphoma(DLBCL).Methods From February 2018 to July 2019,79 cases of DLBCL and 60 cases of reactive hyperplasia lymph node tissues confirmed by pathology in our hospital were selected.The expression level of miR⁃34a was detected by fluorescence quantitative PCR,and the expression level of FOXP1 was detected by Western blot.The correlation between miR⁃34a and FOXP1 was analyzed.The progression free survival of DLBCL patients with different expression of miR⁃34a and FOXP1 was followed up and analyzed.Result The expression level of miR⁃34a in DLBCL tissue was lower than that in reactive proliferative lymph node tissue,and the expression level of FOXP1 was higher than that in reactive proliferative lymph node tissue(P<0.05),and there was a negative correlation between miR⁃34a and FOXP1 in DLBCL tissue;compared with clinical stageⅠ~Ⅱ,normal LDH,IPI score 0⁃1,GCB subtype DLBCL tissue,the expression level of miR⁃34a significantly decreased and the expression level of FOXP1 significantly increased in clinical stageⅢ~Ⅳ,increased LDH,IPI score≥2,non GCB subtype DLBCL tissue.The progression free survival time of DLBCL patients with miR⁃34a expression level≥median was longer than that of DLBCL patients with miR⁃34a expression level<median,and the progression free survival time of DLBCL patients with FOXP1 expression level≥median was shorter than that of DLBCL patients with FOXP1 expression level<median.Conclusion Low expression of miR⁃34a and high expression of FOXP1 are associated with the occurrence,pathological progression and prognosis of DLBCL.