急性脑梗死患者的脂肪酸代谢组学相关性分析

Correlation between free fatty acid metabonomics in patients with acute cerebral infarction

目的应用串联质谱联用法,探讨血浆游离脂肪酸代谢物与急性脑梗死发病的相关性。方法收集2019年10月-2021年1月在锦州医科大学附属第一医院就诊的460例研究对象,根据头部影像学检查是否存在新发脑梗死病灶,分为急性脑梗死组和对照组。采用串联质谱技术对血浆中25个游离脂肪酸代谢物指标进行检测;应用正交偏最小二乘判别分析(OPLS-DA)、受试者工作曲线(ROC)、Logistic回归筛选出两组间的差异代谢物。结果5个游离脂肪酸代谢物和综合性指标W与脑梗死疾病呈相关性。各项代谢物ROC曲线下面积(AUC)分别为:W(0.917)、棕榈酸(0.892)、硬脂酸(0.878)、亚油酸(0.864)、油酸(0.840)、花生四烯酸(0.806)。结论棕榈酸、硬脂酸、亚油酸、油酸、花生四烯酸可能参与了脑梗死的发生与发展,研究其代谢过程可为探究疾病发病机制提供一定依据。

Objective To explore the relationship between free fatty acid metabolites and the acute cerebral infarction based on tandem mass spectrometry.Methods Collecting 460 objects admitted to the first affiliated hospital of jinzhou medical university from October 2019 to January 2020.According to the presence of new cerebral infarction in the head imaging examination,they were divided into two groups:acute cerebral infarction group and control group.The tandem mass spectrometry was used to detect plasma 25 free fatty acids metabolites.Orthogonal partial least squares discriminant analysis(OPLS-DA),Receiver operating characteristic curve(ROC)and logistic regression were used to screen the differential metabolites.Results There are 5 free fatty acid metabolites and the comprehensive indicators W significantly correlated with the incidence of cerebral infarction.The area under curve(AUC)of each metabolite for predicting cerebral infarction was as follows:W(0.917),palmitic acid(0.892),stearic acid(0.878),linoleic acid(0.864),oleic acid(0.840),arachidonic acid(0.806).Conclusion Linoleic acid,palmitic acid,stearic acid,arachidonic acid,oleic acid may be related to the occurrence and development of cerebral infarction,and the study of their metabolic processes may provide a basis for exploring the pathogenesis of cerebral infarction.