癫痫伴发抑郁大鼠杏仁核突触相关蛋白的表达

Expression of synapse-associated protein in epilepsy-associated rats

目的观察癫痫伴发抑郁(EAD)大鼠杏仁核神经生长相关蛋白(GAP-43)、突触素(SYN)、突触后致密蛋白95(PSD95)的表达情况,探讨这3种突触相关蛋白在EAD发病中的作用。方法将健康成年SD大鼠随机分为共病组、癫痫组、抑郁组和正常组。采用氯化锂-匹罗卡品法建立癫痫模型,于造模后第14天对癫痫模型进行抑郁筛选,癫痫伴发抑郁大鼠为共病组;癫痫未伴发抑郁大鼠为癫痫组;抑郁组采用慢性不可预见的中等应激刺激结合孤养法建立大鼠慢性应激抑郁模型;正常组为健康SD大鼠。于造模成功后第29天(4 w后),采用活体灌注固定取脑法取脑,并分离出脑组织中的杏仁核。应用免疫组织化学染色法检测各组大鼠杏仁核GAP43、PSD95及SYN蛋白的表达情况。结果与各对照组相比,共病组GAP-43及PSD95免疫组化阳性细胞数最少,正常对照组最多(P<0.05);其余各组相比差异无统计学意义(P>0.05)。共病组和抑郁组与癫痫组及正常组相比,SYN免疫组化阳性细胞表达均减少(P<0.05);癫痫组较抑郁组增多,较正常组减少(P<0.05)。结论癫痫共病抑郁大鼠杏仁核突触相关蛋白GAP-43、SYN、PSD95免疫阳性细胞表达减少可能与癫痫大鼠抑郁发病有关。

Objective To observe the growth-associated protein-43(GAP-43),synaptophysin(SYN)and postsynaptic density 95(PSD95)in the amygdala of rats with epilepsy-associated depression(EAD),and to explore the role of these three synaptic-related proteins in the pathogenesis of EAD.Methods Healthy adult SD rats were randomly divided into comorbidity group,epilepsy group,depression group and normal group.The lithium chloride-pilocarpine method was used to establish the epilepsy model.The epilepsy model was screened for depression 14 days after the model was established.The rats with epilepsy and depression were the comorbid group;the rats without depression were the epilepsy group.The chronic depression model was established in the depression group by chronic unpredictable moderate stress stimulation combined with orphan method.The normal group is healthy SD rats.On the 29 th day(4 weeks later)after the successful modeling,the brain was taken out by intravital perfusion and fixation in vivo,and the amygdala was separated from the brain tissue.The immunohistochemical staining was used to detect the expression of GAP43,PSD95 and SYN protein in the amygdala of rats in each group.Results Compared with the control groups,the number of GAP-43 and PSD95 immunohistochemical positive cells in the comorbid group was the least,and the normal control group was the most(P<0.05).There was no statistical significance in the other groups(P>0.05).Compared with the epilepsy group and the normal group,the SYN immunohistochemical positive cell expression in the comorbid group and depression group was reduced(P<0.05).The epilepsy group was more than that of the depression group,and less than that of the normal group(P<0.05).Conclusion The decreased expression of GAP-43,SYN,and PSD95 in the amygdala of epileptic rats with depression may be related to the onset of depression in epilepsy rats.