紫檀芪通过TLR4/NF-κB信号通路对肺纤维化大鼠的保护作用

Protecive effect of pterostilbene on pulmonary fibrosis rats through TLR4/NF-kappaB signaling pathway

目的:基于Toll样受体4(TLR4)/核转录因子-κB(NF-κB)信号通路观察紫檀芪(PTE)对博来霉素诱导的肺纤维化大鼠炎症反应和氧化应激的影响。方法:采用气管内注射博来霉素溶液制备大鼠肺纤维化模型。随机分为正常对照组、模型组、紫檀芪组(30 mg/kg)及强的松组(强的松3 mg/kg)。取肺组织进行Masson染色,试剂盒检测羟脯氨酸(HYP)含量、总抗氧化能力(T-AOC)及谷胱甘肽(GSH)表达水平,免疫组化法检测肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)、一氧化氮合酶(iNOS)的含量,Western Blot和PCR检测大鼠肺组织Toll样受体4(TLR4)、核转录因子-κB p65(NF-κB p65)的蛋白和mRNA的表达。结果:与模型组比较,紫檀芪组大鼠肺组织中HYP、TNF-α、IL-1β、iNOS蛋白表达量下降,T-AOC、GSH表达增强,TLR4和NF-κB p65的蛋白和mRNA表达水平明显下调。结论:紫檀芪能有效缓解肺纤维化,其机制可能与通过调控TLR4/NF-κB信号通路,抑制肺纤维化过程中的炎性因子、减弱氧化应激反应有关。

Objective: To observe the effect of pterostilbene(PTE) on pulmonary fibrosis model rats.Methods: The rats were randomly divided into normal control group, model group, PTE group(PTE30 mg/kg), and prednisone group(prednisone 3 mg/kg). The rat pulmonary fibrosis model was prepared by intratracheal injection of bleomycin solution. Masson staining was performed on lung tissue.The total antioxidant capacity(T-AOC), hydroxyproline(HYP), and glutathione(GSH) expression levels in lung tissue were detected. TNF-α, IL-1β, and iNOS levels were detected by immunohistochemistry. Protein and mRNA levels of TLR4 and NF-κB p65 were detected by Western Blot and PCR respectively. Results: Compared with those in model group, the expression levels of HYP,TNF-α, IL-1β, and iNOS were decreased, T-AOC and GSH were increased, and the protein and mRNA levels of TLR4 and NF-κB p65 were significantly decreased. Conclusion: PTE can effectively alleviate pulmonary fibrosis, and its mechanism may be related to regulating TLR4/NF-κB pathway, inhibiting inflammatory factors in the process of pulmonary fibrosis, and weakening oxidative stress response.