不同窖龄及位置窖泥微生物群落和代谢组分的差异

Difference of the microbial community and metabolite in pit mud with different age and position

基于多相检测技术探究了不同窖龄窖泥微生物群落及主要代谢组分的时空特点。结果表明,窖池微环境和代谢物间相互影响,胁迫微生物群落定向进化。Caproiciproducens和Clostridium sensu stricto 12等7种细菌与窖龄相关,产甲烷古菌则与窖泥的位置有关。此外,不锈钢代替窖泥盖,降低了窖池中Lactobacillus和Nitrosopumilaceae的丰度。窖池微环境和群落间互作共同影响群落组成,Caproiciproducens、Clostridium sensu stricto 12、Methanobacterium和Methanoculleus与己酸酯呈正相关。荧光原位杂交定量检测的结果表明,梭菌属等功能菌群随窖龄的增长而增高,且在窖底泥中含量更高。

The time-space characteristics of microbial communities and major metabolic components in pit mud were explored by multiphase detection technology. The results suggested that pits′ microenvironment and flavored metabolites influenced each other, which led to the directed evolution of microbial communities. Seven of genera, including Caproiciproducens, Clostridium sensu stricto 12, were related with age, while methanogen was associated with location. Besides, the abundance of Lactobacillus and Nitrosopumilaceae was decreased as sealing the cellar by stainless steel instead of pit mud. Community composition was not only affected by the microenvironment of pits, but also by community interaction. Caproiciproducens, Clostridium sensu stricto 12, Methanobacterium and Methanoculleus were positively correlated with caproate ester. The quantitative detection results of fluorescence in situ hybridization uncovered that the functional flora such as Clostridium were increased with the increasing of pit age, and was higher in the bottom of the cellar.