初情期母牦牛乏情的产生机理探讨

Study on the Mechanism of Anestrus in Female Yak During Proestrus

青藏高原地区母牦牛繁殖率普遍偏低,初情期母牦牛的乏情情况较为普遍,严重影响其繁殖性能。为探究初情期母牦牛乏情产生的机理,以其营养代谢为切入点,对乏情和发情的初情期母牦牛的血液各类指标进行了检测。结果表明,乏情组母牦牛促性腺激素(GnRH)、促卵泡素(FSH)、促黄体素(LH)、雌激素(E_(2))和孕酮(P_(4))均显著低于发情组(P<0.05),游离脂肪酸(NEFA)、葡萄糖(GLU)也显著低于发情组(P<0.05),而总蛋白(TP)在两组之间没有显著性差异(P>0.05);另外,在相关性的研究中发现,NEFA与GnRH、E_(2)和P_(4)存在显著性相关(P<0.05),GLU与GnRH和E_(2)之间也存在显著相关性(P<0.05);通过信号通路数据库的搜索和调控网络的整合,阐述了营养代谢引起乏情的产生过程,发现脂类代谢和糖代谢是能够影响和促进母畜发情的。综上,初情期母牦牛脂类代谢和糖类代谢与生殖激素的分泌相关,也可能是引起乏情的原因之一。

The reproductive rate of female yaks in Qinghai Tibet Plateau is generally low,and the lack of estrus in female yaks is common during early estrus,which affects its reproductive performance seriously.To investigate the mechanism of anestrus of female yak in puberty,the blood index of anestrus and estrus female yaks in puberty based on the nutrition metabolism were detected.The results showed that the GnRH,FSH,LH,E_(2) and P_(4) in the anestrus group were significantly lower than those in the estrus group(P<0.05),NEFA and Glu were also significantly lower than those in the estrus group(P<0.05),while TP had no significant difference between the two groups(P>0.05).On the other hand,it was found that NEFA was significantly correlated with GnRH,E_(2) and P_(4)(P<0.05),and there was a significant correlation between Glu and GnRH,E_(2)(P<0.05).The mechanism of anestrus caused by nutritional regulation was clarified,according to the search of signaling pathway database and the integration of regulatory network.Above all,it was found that the lipid metabolism and carbohydrate metabolism were related to the secretion of reproductive hormone,which might be one of the causes of estrus.