阿萨尔基亚芽孢杆菌对大丽轮枝菌微菌核的抑制作用

Inhibition Effect of Bacillus axarquiensis TUBP1 on MicrosclerotiaGermination of Verticillium dahliae Kleb

旨在分析阿萨尔基亚芽孢杆菌(TUBP1)对大丽轮枝菌(犞犲狉狋犻犮犻犾犾犻狌犿犱犪犺犾犻犪犲)微菌核的抑制作用。通过菌核萌发法、共培养、盆栽试验和扫描电镜观察,明确阿萨尔基亚芽孢杆菌发酵液对微菌核萌发的影响。菌核萌发试验结果表明,大丽轮枝菌微菌核经过阿萨尔基亚芽孢杆菌原发酵液、5倍和10倍稀释发酵液处理72h后,微菌核萌发率分别为62.52%、70.10%、75.33%;共培养结果显示,施加原浓度发酵液后大丽轮枝菌微菌核形成减少,由菌核型转变为菌丝型,且原浓度发酵液抑菌率达到64.69%;扫描电镜观察,经阿萨尔基亚芽孢杆菌原浓度发酵液处理后,分生孢子及微菌核形成减少,且随培养时间延长菌丝发生膨胀、断裂,出现异型,大丽轮枝菌自身形态结构被破坏。盆栽试验结果表明,对照组和处理组微菌核萌发率分别为61.15%和37.33%。

Inhibition effect of Bacillus a.carquiensis TUBP1 on microsclerotia germination of Verticil-lium dahliae were analyzed in this paper.Fermentation broth of B.a.carquiensis TUBP1 acting on mi-crosclerotia germination of V.dahliae were detected by microsclerotia germination method,pot ex-periment and scanning electron microscope.The results showed that after inoculation with B.a.carquiensis TUBP1 original fermentation broth for 72 hours,5-fold and 10-fold diluted fermentationbroth,the germination rates of microsclerotia were 62.52%,70.10%and 75.33%,respectively.Coculture showed that the microsclerotia formation of V.dahliae in the original concentration fermentation broth decreased and the inhibition rate reached to 64.69%.Besides,the type of V.dahliaechanged from sclerotium to mycelium.In a greenhouse study,the microsclerotia germination rates ofthe control group and the treatment group were 61.15%and 37.33%,respectively.Under treatmentwith the original fermentation broth,the microsclerotia germination was lower than that of the controlgroup.The experimental results prove that B.acarquiensis TUBP1 has a certain inhibitory effect onmicrosclerotia germination of V.dahliae.