谷子矮秆材料萌发期对赤霉素的敏感性分析

Sensitivity of Millet Dwarf Materials to Gibberellin During Germination

为明确谷子矮秆材料萌发期的赤霉素敏感性反应类型,选用龙谷30×胜谷的杂交组合后代品系JA1~JA10、A1~A10、ZA1~ZA10等30份矮秆材料进行萌发期不同浓度赤霉素处理,分析其根长、中胚轴长、胚芽鞘长、胚芽长以及叶长对GA_(3)的敏感性。结果表明:在两种赤霉素浓度处理条件下,60,80及110 cm株系分离后代各性状对GA_(3)敏感性差异不显著。在GA_(3)敏感性材料中,胚芽的敏感性要强于中胚轴、根、胚芽鞘和叶,且胚芽的敏感性都达到极显著水平以上;其次是中胚轴的敏感性要强于根、胚芽鞘和叶。因此,用GA_(3)鉴定谷子敏感性时,胚芽和中胚轴可以作为鉴定的首选器官。在鉴定GA_(3)敏感性所利用的5个性状中,根长可能会随着赤霉素的处理而减小,说明赤霉素处理可能会对根长产生抑制作用,而其他性状均随着赤霉素处理而增加,即适宜浓度的赤霉素处理对中胚轴长、胚芽鞘长、胚芽长和叶长产生促进作用。本试验利用的30份材料均对GA_(3)处理敏感,表现在不同器官的伸长或抑制,由此推断这些矮秆材料可能含有与GA_(3)合成相关基因,这些材料的矮化可能是由于GA_(3)合成基因发生突变,导致植株不能合成正常生长所需的GA_(3)。

In order to clarify the gibberellin sensitivive reaction type of millet dwarf materials at germination stage,30 dwarf materials,which used Longgu 30 as female parent and Shenggu as male parent,dwarf lines JA1-JA10,A1-A10 and ZA1-A10,were treated with gibberellin at different concentrations during germination.The sensitivity of root length,hypocotyl length,coleoptile length,germ length and leaf length to GA_(3)were analyzed.The results showed that under the treatment of two gibberellin concentrations,there was no significant difference in the sensitivity of the offspring of 60,80 and 110 cm line to GA_(3).Among the GA_(3)sensitive materials,the sensitivity of germ was stronger than that of hypocotyl,root,coleoptile and leaf,and the sensitivity of germ reached a very significant level.Secondly,the sensitivity of mesodermal axis was stronger than that of root,coleoptile and leaf.Therefore,when using GA_(3)to identify millet sensitivity,germ and mesodermal axis can be used as the preferred organs for identification.Among the five traits used to identify GA_(3)sensitivity,root length may decrease with gibberellin treatment,indicating that gibberellin treatment may inhibit root length,while other traits increase with gibberellin treatment.That is,gibberellin treatment with appropriate concentration canpromote mesodermal axis length,coleoptile length,germ length and leaf length.The 30 materials used in this experiment were sensitive to GA_(3)treatment,which were manifested in the elongation or inhibition of different organs.It was inferred that these dwarf materials may contain genes related to GA_(3)synthesis.The dwarfing of these materials may be due to the mutation of GA_(3)synthesis gene,resulting in the plant unable to synthesize GA_(3)required for normal plant growth.