摘要
It is well known that fossil fuels are one of the major pollutants that contribute to climate change and there is a need to develop alternatives to their uses.One option is the use of a renewable fuel such as biogas obtained from the breaking down of organic matter in the anaerobic digestion process.When biogas is cleaned up to a certain quality,it can substitute the use of natural gas.However,this slow process requires optimization to increase the amount of gas.There are chemical,physical and biological treatments for organic matter to increase the amount and quality of biogas.In the former research,a biological treatment was tested by adding a selection of microorganisms that showed hydrolytic activity to an anaerobic digester for its optimization.First,a selection of microorganisms with the ability to grow in fresh sewage sludge as only nutrient was selected.Then,the lipolytic,cellulolytic and PA(Proteolytic Activity)of microorganisms was tested using selective growth media.Finally,10 samples with the greatest hydrolytic activity were used to evaluate their effects in the increasing of biogas production in the anaerobic process.The results showed that 83%of the microorganisms with the ability to grow in sewage sludge were proteolytic,while the percentage of lipolytic and cellulolytic microorganisms was smaller.Furthermore,it was found that proteolytic microorganisms are the ones that increase biogas yield in a maximum of 80%with a COD(Chemical Oxygen Demand)reduction of 58%.
