基于网络药理学的泽泻治疗2型糖尿病作用机制研究

Research on the action mechanisms of oriental waterplantain rhizome in the treatment of type 2 diabetes mellitus based on network pharmacology

目的基于网络药理学对泽泻抗2型糖尿病(T2DM)作用机制的研究。方法应用Swiss Target Prediction获取泽泻潜在靶点,筛选与T2DM有关基因靶点。构建PPI网络筛选关键成分和关键靶点,运用DAVID进行GO功能分析及KEGG通路分析。结果获知泽泻抗T2DM潜在靶点48个,关键靶点主要包括PRK3CA、AKT1、RPS6KB1等。泽泻抗T2DM与细胞迁移、代谢、信号转导、受体激活等生物学过程有关;泽泻通过与糖尿病并发症有关的ErbB信号转导通路、胰岛素抵抗通路和胰岛素信号转导通路等协调发挥直接或间接治疗作用。结论预测泽泻抗T2DM的作用靶点和潜在作用机制,为下一步实验研究提供科学依据。

Objective To investigate the action mechanisms of(Rhizoma Alismatis)in the treatment of type 2 diabetes mellitus(T2DM)based on network pharmacology.Methods The potential targets of oriental waterplantain rhizome were obtained by Swiss Target Prediction,and the gene targets related to T2DM were screened.PPI network was constructed to screen key components and key targets,and DAVID was used to analyze GO function and KEGG pathway.Results It was known that there were 48 potential targets of oriental waterplantain rhizome in the treatment of T2DM,and the key targets mainly included PRK3CA,AKT1,RPS6KB1,etc.In the treatment of T2DM,oriental waterplantain rhizome was related to biological processes such as cell migration,metabolism,signal transduction and receptor activation.Oriental waterplantain rhizome played a direct or indirect therapeutic role through coordination of ErbB signal transduction pathway,insulin resistance pathway and insulin signal transduction pathway related to diabetic complications.Conclusion The targets and potential action mechanisms of oriental waterplantain rhizome in the treatment of T2DM are predicted,and they provide scientific basis for the next experimental research.