摘要
研究应用正交设计筛选处方制备甘草次酸脂质体,注入乙醇后,应用葡聚糖凝胶G-50柱对脂质体及游离药物予以分离,采用HPLC法对包封率予以测定,通过对脂质体外观形态、粒径及zeta电位等的观察,总结脂质体脂肪规律。结果显示脂质体包封率为(91.53±2.34)%,形态以球形、类球形为主,体外释放与Higuchi方程相符,稳定性好。实践证实该方法下所制备的甘草次酸脂质体处方工艺合理,稳定性好,体外缓释释放。
The study used orthogonal design to screen the prescription to prepare glycyrrhetinic acid liposomes.After injecting ethanol,the liposomes and free drugs were separated using Sephadex G-50 column,and the encapsulation efficiency was determined by HPLC.,By observing the appearance,particle size and zeta potential of liposomes,summarize the fat law of liposomes.The results showed that the liposome encapsulation efficiency was(91.53±2.34)%,and the shape was mainly spherical and quasi-spherical.The in vitro release was consistent with the Higuchi equation,and the stability was good.Practice has proved that the glycyrrhetinic acid liposomes prepared by this method are reasonable in prescription,good stability,and sustained release in vitro.
作者
张素红
周敬
李阳杰
刘影
Zhang Su-hong;Zhou Jing;Li Yang-jie;Liu Ying
出处
《化工设计通讯》
CAS
2022年第2期97-99,共3页
Chemical Engineering Design Communications
基金
教育部产学合作协同育人项目(202101021046)。
关键词
甘草次酸脂质体
正交设计
包封率
体外释放
glycyrrhetinic acid liposomes
orthogonal design
encapsulation efficiency
in vitro release
