桃叶珊瑚苷对高糖诱导的肾小管上皮细胞炎症损伤的机制

The mechanism of aucubin up-regulating the expression of miR-374 to inhibit high glucose-induced inflammatory injury of renal tubular epithelial cells

目的探讨桃叶珊瑚苷(AU)对高糖诱导的肾小管上皮细胞炎症损伤的影响及分子机制。方法将肾小管上皮细胞HK-2分为对照(Con)组、高糖(HG)组、桃叶珊瑚苷低、中、高浓度(HG+AU-L、M、H)组、HG+miR-NC组、HG+miR-374组、HG+AU+anti-miR-NC组、HG+AU+anti-miR-374组。酶联免疫吸附试验(ELISA)检测IL-6、TNF-α水平;流式细胞术检测细胞凋亡;实时荧光定量PCR(RT-qPCR)检测miR-374表达水平。结果高糖诱导的肾小管上皮细胞中IL-6、TNF-α水平升高,细胞凋亡率升高,miR-374表达水平降低(P<0.05)。不同浓度桃叶珊瑚苷处理后,IL-6、TNF-α水平降低,细胞凋亡率降低,miR-374表达水平升高,呈浓度依赖性(P<0.05)。miR-374过表达后,高糖诱导的肾小管上皮细胞中IL-6、TNF-α水平降低,细胞凋亡率降低(P<0.05)。抑制miR-374表达逆转了桃叶珊瑚苷对高糖诱导的肾小管上皮细胞炎症损伤的作用。结论桃叶珊瑚苷通过上调miR-374表达抑制高糖诱导的肾小管上皮细胞炎症损伤。

Objective To investigate the effect of aucubin on the inflammatory injury of renal tubular epithelial cells induced by high glucose and its molecular mechanism.Methods Renal tubular epithelial cells HK-2 were divided into control(Con)group,high glucose(HG)group,aucubin low,medium and high concentration(HG+AU-L,M,H)groups,and HG+miR-NC Group,HG+miR-374 group,HG+AU+anti-miR-NC group,HG+AU+anti-miR-374 group.Enzyme-linked immunosorbent assay(ELISA)was used to detect IL-6 and TNF-αlevels.The flow cytometry was used to detect cell apoptosis.The real-time fluorescent quantitative PCR(RT-qPCR)was used to detect miR-374 expression level.Results The levels of IL-6 and TNF-αin renal tubular epithelial cells induced by high glucose were increased,the rate of apoptosis was increased,and the expression level of miR-374 was decreased(P<0.05).After treatment with different concentrations of aucubin,the levels of IL-6 and TNF-αwere decreased,the rate of apoptosis was decreased,and the expression level of miR-374 was increased,in a concentration-dependent manner(P<0.05).After the overexpression of miR-374,the levels of IL-6 and TNF-αin renal tubular epithelial cells induced by high glucose were decreased,and the apoptosis rate was decreased(P<0.05).Inhibition of miR-374 expression reversed the effect of aucubin on the inflammatory injury of renal tubular epithelial cells induced by high glucose.Conclusion Aucubin inhibits the inflammatory injury of renal tubular epithelial cells induced by high glucose by regulating the expression of miR-374.