应用Cephodex微载体悬浮培养DF-1细胞增殖水貂犬瘟热病毒

The proliferation of mink canine distemper virus by suspension culture of the DF-1 cells with Cephodex microcarrier

为准确把握Cephodex微载体及其悬浮培养技术在水貂犬瘟热病毒增殖中的特点优势,本试验对微载体培养与单层静置培养2种方法中的细胞密度与病毒滴度进行了检测,同时对5,10,15 g/L 3种不同微载体质量浓度下的细胞生长与病毒增殖效果作了比较分析。结果显示,微载体培养法与单层静置培养法比较,DF-1细胞密度可提高3倍以上,CDV3滴度能提高10^(0.5)TCID_(50)/0.1 mL;而且,10 g/L微载体质量浓度时的细胞密度较5 g/L时提高约2倍,CDV3滴度可提高10^(0.3)TCID_(50)/0.1 mL;进一步增加微载体质量浓度至15 g/L,细胞密度仅增加12%,而CDV3滴度几乎不变。结果表明,微载体培养法用于CDV3高效增殖明显优于单层静置培养法,用于CDV3增殖的最适Cephodex质量浓度为10 g/L。

In order to accurately grasp the characteristics and advantages of Cephodex microcarrier suspension culture technology in the process of canine distemper virus proliferation,the DF-1 cells density and the CDV3 titer were measured in the two methods of microcarrier culture and static monolayer culture,and the growth of DF-1 cells and CDV3 proliferation under concentrations of 5,10,15 g/L Cephodex were compared and analyzed.The results showed that the density of DF-1 cells increased by more than 3 times and the CDV3 titer increased 10^(0.5)TCID_(50)/0.1 mL/0.1 mL by microcarrier culture method compared with the static monolayer culture.Moreover,the DF-1 cells density was increased about 2-fold and CDV3 titer increased by 10^(0.3)TCID_(50)/0.1 mL at 10 g/L Cephodex concentration compared with that at 5 g/L.When the concentration of microcarrier was further increased to 15 g/L,the cell density was only increased by 12%,while the CDV3 titer was almost unchanged.Therefore,for the efficient proliferation of CDV3,the effect of suspension culture with Cephodex is better than that of monolayer culture,and the optimal concentration of Cephodex is 10 g/L.