摘要
目的探索振动刺激诱发前庭诱发电位(VsEP Vestibular Evoked Potential)检测系统的构建及电位记录方法。旨在建立一套操作简便,无创伤,可长期反复操作,结果稳定的前庭诱发电位检测系统。方法选取健康小鼠6只,随机分为健康对照组(3只)和庆大霉素造模组(3只)。使用振动刺激诱发记录前庭诱发电位(Vestibular evoked potential,VsEP),并与声刺激诱发的听性脑干反应(ABR)进行比较。结果6只小鼠均能引出稳定的VsEP波。庆大霉素造模组在SPLR波形振动刺激下比较健康对照组阈值升高(P<0.01),N1-P1振幅降低(P<0.05)。ABR阈值无明显差异。结论振动刺激诱发的前庭诱发电位检测系统是一种操作简便、无创伤、波形稳定的动物前庭功能检测系统,可用于小鼠前庭功能电生理状态的客观评价研究。
Objective To develop a testing system and protocols for vibration elicited vestibular sensory-evoked potentials(VsEPs)that are non-invasive,stable and easy to use with reproducible results for animal studies.Method Six healthy mice were selected to be in a gentamicin treatment or control group(n=3 in each group)and received VsEP and click elicited ABR tests.Result Stable VsEP waves could be elicited in all 6 mice with typical waves,although with significantly higher thresholds and lower amplitudes in the gentamicin group than in the control group under SPLR stimulation(P<0.01 and P<0.05,respectively).Conclusion A vibration-elicited VsEP test system is easy to set up and non-invasive,and can produce repeatable and stable waveforms reflecting vestibular function,which can be widely used in objective vestibular electrophysiological evaluation in mice.
作者
高滢
张青
鸭頭辉
山岨達也
岩崎真一
张滟
马伟军
陈耔辰
张玉忠
许珉
任晓勇
GAO Ying;ZHANG Qing;Kamogashira Teru;Yamasoba Tatsuya;Iwasaki Shinichi;ZHANG Yan;MA Weijun;CHEN Zichen;ZHANG Yuzhong;XU Min;REN Xiaoyong(Department of Otolaryngology Head and Neck Surgery,Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an,710004,China;Department of Otolaryngology Head and Neck Surgery,Faculty of Medicine University of Tokyo,Tokyo,1130034,Japan;Department of Otolaryngology&Head and Neck Surgery,Xinhua Hospital,Shanghai Jiaotong University School of Medi‐cine,Ear Institute,Shanghai Jiaotong University School of Medicine,Shanghai Key Laboratory of Translation Medicine on Ear and Nose Diseases,Shanghai,200092,China;Department of Otolaryngology,Head and Neck Surgery,Graduate School of Medical Sciences,Nagoya City University,Nagoya,467-8601,Japan)
出处
《中华耳科学杂志》
CSCD
北大核心
2022年第1期111-115,共5页
Chinese Journal of Otology
基金
国家自然科学基金项目(82101224,81970891,81670945)
陕西省重点研发计划项目(2019SF-182)
陕西省国际合作重点项目(2020KWZ-19)
院基金自由探索项目(2020YJ(ZYTS)043)。
关键词
前庭诱发电位
耳石器
小鼠
Vestibular Evoked Potential
Otolith
Mouse
