沈阳红药胶囊HPLC指纹图谱及聚类分析研究

HPLC Fingerprint and Cluster Analysis of Shenyang Hongyao Capsules

目的:建立沈阳红药胶囊HPLC指纹图谱,并对其进行聚类分析研究。方法:采用Agilent C_(18)(250 mm×4.6 mm,5μm)色谱柱,以水-乙腈为流动相进行梯度洗脱,流速为1.0 ml·min^(-1),柱温为25℃,检测波长为210 nm,进样量为10μl。结果:10批沈阳红药胶囊HPLC指纹图谱确定10个共有峰,各批供试品相似度均在0.9以上。10批供试品可聚为2类,S1、S2、S3、S4为Ⅰ类,其余供试品为Ⅱ类,再对Ⅱ类供试品进行细化归类,S5、S7、S8可归为一类,S6、S9、S10归为另一类。结论:所建方法准确、专属性强,可有效评价沈阳红药胶囊质量。

Objective:To establish the HPLC fingerprint of Shenyang Hongyao capsules,and carry out the cluster analysis research.Methods:An Agilent C_(18)(250 mm×4.6 mm,5μm)chromatographic column was used for gradient elution with acetonitrile-water as the mobile phase.The flow rate was 1.0 ml·min;,the column temperature was 25℃,the detection wavelength was 210 nm and the injection volume was 10μl.Results:Ten common peaks were determined by the HPLC fingerprint of 10 batches of Shenyang Hongyao capsules,and the similarity of each batch was above 0.9.The 10 batches of samples could be grouped into 2 categories,S1,S2,S3 and S4 were classified into classⅠ,and the rest of samples were classified into classⅡ.The samples in classⅡwere classified in detail,and S5,S7 and S8 could be classified into one category,while S6,S9 and S10 could be classified into another category.Conclusion:The method is accurate and specific,which can evaluate the quality of Shenyang Hongyao capsules effectively.