野生蘑菇中多种元素含量及砷、硒形态分析技术研究

Study on the content of various elements and speciation analysis technology of arsenic and selenium in wild mushrooms

目的分析野生蘑菇中多种元素及砷、硒形态含量,对分析方法进行研究讨论,并对野生蘑菇中砷、硒元素含量和形态占比进行探讨。方法采用微波消解-电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry, ICP-MS)对我国不同区域代表性可食用野生蘑菇的多种元素进行分析,针对砷、硒含量较高的样品,采用高效液相色谱-原子荧光光谱法(high performance liquid chromatography-atomic fluorescence spectrometry, HPLC-AFS)/ICP-MS进行砷、硒元素形态分析,对前处理条件和检测条件进行研究和优化,并对结果进行比较分析。结果电感耦合等离子体质谱法检测野生蘑菇中多种元素的分析方法线性关系良好,各元素检出限在0.001~0.200 mg/kg之间,回收率为96.8%~102.5%;采用HPLC-AFS/ICP-MS可将蘑菇中砷酸根、亚砷酸根、硒酸根、亚硒酸根等不同元素形态进行有效地分离和检测,方法线性关系良好,回收率为95.2%~101.6%。结论本方法前处理简便、灵敏度高、干扰小、精密度高、准确性好,适合于检测蘑菇中多种元素含量及其形态分析,并为科学评价野生蘑菇中常见元素含量提供数据参考。

Objective To analyze the content of various elements,arsenic and selenium in wild mushrooms,study and discuss the analytical methods,and discuss the content and proportion of arsenic and selenium elements in wild mushrooms.Methods A variety of elements in representative edible wild mushrooms in different regions of China were analyzed by microwave digestion-inductively coupled plasma mass spectrometry(ICP-MS).For samples with high content of arsenic and selenium,the speciation of arsenic and selenium was analyzed by high performance liquid chromatography-atomic fluorescence spectrometry(HPLC-AFS)/ICP-MS,the pretreatment and detection conditions were studied and optimized,and the results were compared and analyzed.Results The method of ICP-MS for the determination of various elements in wild mushrooms had good linearity relationship,the limits of detection of each element were between 0.001-0.200 mg/kg,and the recoveries were 96.8%-102.5%;the forms of arsenate,arsenite,selenite and selenite in wild mushrooms could be effectively separated and detected by HPLC-AFS/ICP-MS.The linear relationships of the method were good,and the recoveries were 95.2%-101.6%.Conclusion This method has the advantages of simple pretreatment,high sensitivity,low interference,high precision and good accuracy.It is suitable for the determination of various element content and morphological analysis in mushrooms,and provides data reference for the scientific evaluation of common element content in wild mushrooms.