光学成像技术活体评价PAEs促皮损愈合的药效

In Vivo Evaluation of the Efficacy of PAEs in Promoting Skin Wound Healing by Optical Imaging Technique

利用双光子激发荧光成像技术、二次谐波成像技术与毛细血管镜系统,活体评估鹿茸蛋白粗提物给药后创面愈合情况。提取鹿茸中水溶性总蛋白,质谱鉴定其成分。全层切除皮肤制备皮肤创伤模型大鼠,于给药10 d后活体观测创面表皮厚度、真皮胶原纤维的含量情况及毛细血管的生长情况。结果发现:蛋白含量为61.29%,质谱鉴定出135种蛋白,18种氨基酸。给药10 d后,中高剂量组创面收缩明显,表皮厚度增加,新生胶原纤维增多;各给药组较模型组毛细血管网数有增加趋势;高剂量组较模型组血流量显著下降,血流趋于稳定。以上三种方法综合评估全层切除大鼠皮肤愈合过程中的药效学作用,提示中高剂量组具有显著促进血管生长及胶原纤维生长的作用,可以提高愈合质量。

This study is to use two-photon excitation fluorescence imaging technology,second harmonic imaging technology and capillary angioscope system to evaluate wound healing after administration of crude velvet protein extract.To this end,the water-soluble total protein from deer antler was extracted and its components by mass spectrometry were identified.Full-thickness excision of the skin to prepare skin trauma model rats,10 days after the administration of the drug,the changes in the thickness and morphology of the dermis and epidermis of the rats and the growth of capillaries in the wound surface were observed in vivo.It has found that the protein content was 61.29%.Mass spectrometry identified 135 proteins and 18 amino acids.After 10 days of administration,the wounds in the medium and high dose group shrank significantly,the epidermal thickness increased,and the number of new collagen fibers increased.Compared with the normal skin,the capillary network and the number of free capillaries in each administration group were significantly reduced,and the capillary network number of the model group increased;The blood flow of the high-dose group was significantly lower than that of the model group,and the blood flow tended to be stable.The above three methods comprehensively evaluate the pharmacodynamic effects of the full-thickness excision in the skin healing process of rats.The results indicate that the middle and high dose group can significantly promote the growth of blood vessels and collagen fibers,and can improve the quality of healing.