露地条件下高效氟氯氰菊酯和噻虫胺在甘蓝中的残留行为与膳食风险评估

Residues behavior and dietary risk assessment of beta-cyfluthrin and clothianidin in cabbage under open-field conditions

目的探究新型复配制剂42%高效氟氯氰菊酯·噻虫胺悬浮剂在甘蓝上应用后的残留行为,并评估其膳食摄入风险。方法甘蓝样品中的高效氟氯氰菊酯和噻虫胺采用乙腈提取,N-丙基乙二胺和多壁碳纳米管净化,经高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)分析,采用基质匹配外标校准法定量。结果高效氟氯氰菊酯和噻虫胺在甘蓝中的平均回收率在80%~104%之间,相对标准偏差(relative standard deviations,RSDs)不大于11.2%。两种化合物的定量限(limits of quantification,LOQs)均为0.01 mg/kg。该制剂在中国12个地区以94.5 g a.i./hm;的剂量在甘蓝上施用两次,间隔10 d。在推荐的安全间隔期(pre-harvest interval,PHI,7 d)采摘的甘蓝中高效氟氯氰菊酯和噻虫胺的最终残留量分别小于等于0.44和0.32 mg/kg。结合我国膳食结构可得这两个化合物在不同人群中的慢性和急性风险商介于1.1%~52.7%之间。结论在推荐的良好农业规范(good agricultural practice,GAP)条件下使用该制剂不会对不同年龄段的消费者造成膳食摄入风险。

Objective To explore the residue behavior of the new mixed formulation 42%betacyfluthrin·clothianidin suspension concentrate on cabbage,and assess the dietary intake risks.Methods Beta-cyfluthrin and clothianidin in cabbage samples were extracted by acetonitrile,purified by primary secondary amine and multi-walled carbon nanotube,and analyzed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),and quantified by matrix matching external standard calibration method.Results The average recoveries of these 2 kinds of compounds in cabbage were between 80%and 104%,and the relative standard deviations(RSDs)were no more than 11.2%.The limits of quantification(LOQs)were 0.01 mg/kg for the both compounds.The formulation was sprayed twice on the cabbage at a dose of 94.5 g a.i./hm;with an interval of 10 d in 12 typical areas of China.The terminal residues of beta-cyfluthrin and clothianidin in the cabbage picked at the recommended pre-harvest interval(PHI,7 d)were less than or equal to 0.44 and 0.34 mg/kg,respectively.Combined with the China’s dietary pattern,the chronic and acute risk quotients of these two compounds were ranged from 1.1%to 52.7%in different populations.Conclusion The application of the formulation under the recommended good agricultural practice(GAP)conditions can not cause dietary intake risks to consumers of different age groups.