PRiME HLB固相萃取柱结合气相色谱-串联质谱法快速测定茶叶中200种农药残留

Rapid determination of 200 kinds of pesticide residues in tea by PRiME HLB solid phase extraction column combined with gas chromatography-tandem mass spectrometry

目的建立PRiMEHLB固相萃取柱结合气相色谱-串联质谱法(gas chromatography-tandem mass spectrometry,GC-MS/MS)快速测定茶叶中200种农药残留量的分析方法。方法样品用乙腈提取,经PRiME HLB通过式固相萃取柱净化,残留量采用气相色谱-串联质谱法进行检测。结果200种农药在各自质量浓度范围内均呈现良好线性关系,相关系数(r^(2))均大于等于0.995,方法定量限在0.01~0.05mg/kg之间。在阴性茶叶中加入低、中、高3个添加水平,平均加标回收率及相对标准偏差分别为70.0%~129.2%、1.0%~23.7%。结论该方法操作便捷、快速、准确性强,适合于茶叶中农药多残留高通量的筛查。

Objective To establish a method for the rapid determination of 200 kinds of pesticide residues in tea by PRiME HLB solid phase extraction column combined with gas chromatography-tandem mass spectrometry(GC-MS/MS).Methods The sample was extracted with acetonitrile,purified by PRi ME HLB solid phase extraction column,and the residues were detected by gas chromatography-tandem mass spectrometry.Results Two hundred kinds of pesticides showed good linear relationships in their respective mass concentration range,the correlation coefficients(r^(2))were greater than or equal to 0.995,and the limits of quantification of the method were 0.01-0.05 mg/kg.Adding low,medium and high addition levels to negative tea,The average recoveries and relative standard deviations were 70.0%-129.2%and 1.0%-23.7%respectively.Conclusion This method is easy to operate,rapid and has strong accuracy,which is suitable for high-throughput screening of pesticide multi-residues in tea.