沙眼衣原体肺炎患儿痰载菌量与病情严重程度相关性研究

Correlation between Sputum Bacterial Load and Disease Severity in Children with Chlamydia Trachomatis Pneumonia

目的:探讨沙眼衣原体肺炎患儿痰载菌量与病情严重程度的相关性。方法:选取147例沙眼衣原体肺炎患儿为研究对象,采用荧光定量聚合酶链反应技术对标本中沙眼衣原体DNA进行定量检测,根据检测结果分为低拷贝数组(<10^(4)/mL)、中拷贝数组(10^(4)~10^(6)/mL)和高拷贝数组(>10^(6)/mL),比较不同组别间的临床症状、胸片结果、呼吸机辅助呼吸时间及住院时间的差异,出院2周后复查胸片,并与住院期间胸片结果进行比较。结果:气促时间比较,高拷贝数组与中、低拷贝数组之间差异有统计学意义(P<0.01),中、低拷贝数组之间差异无统计学意义(P>0.05);热程比较,高拷贝数组与中拷贝数组(P<0.05)、低拷贝数组(P<0.01)之间差异有统计学意义,中、低拷贝数组之间差异无统计学意义(P>0.05);胸片结果比较,高拷贝数组与中、低拷贝数组之间差异有统计学意义(P<0.05),中、低拷贝数组之间差异无统计学意义(P>0.05);住院时间比较,高拷贝数组与中、低拷贝数组之间差异有统计学意义(P<0.01),中、低拷贝数组之间差异无统计学意义(P>0.05);3组患儿呼吸机辅助呼吸时间及发热例数比较差异无统计学意义(P>0.05)。两周后胸片恢复情况均较好,3组比较差异无统计学意义(P>0.05)。结论:沙眼衣原体肺炎痰中载菌量达到一定数量后与患儿气促时间、热程、胸片严重程度、住院时间等临床特征有一定相关性,但是预后较好。

Objective:To probe into the correlation between sputum bacterial load and disease severity in children with Chlamydia trachomatis pneumonia.Methods:C.trachomatis DNA was quantitatively detected by fluorescence quantitative polymerase chain reaction(PCR)sputum from 147 children with C.trachomatis pneumonia.The 147 cases were classified into low load group(<10^(4)/mL),medium load group(from 10^(4) to 10^(6)/mL)and high load group(>10^(6)/mL).Differences of clinical symptoms,results of chest radiography,duration of mechanical ventilation and length of stay were compared among different groups.Chest radiography were reviewed two weeks after discharge and compared with the results of chest radiography during hospitalization.Results:There was statistically significant difference in the time of shortness of breath among the high load group,the medium load group and the low load group(P<0.01),yet there was no statistically significant difference between the medium load group and the low load group(P>0.05).There were statistically significant differences in the duration of fever between the high load group and the medium load group(P<0.05),the high load group and low load group(P<0.01),yet there was no statistically significant difference between the medium load group and the low load group(P>0.05).There was statistically significant difference in the results of chest radiography among the high load group,the medium load group and the low load group(P<0.05),yet there was no statistically significant difference between the medium load group and the low load group(P>0.05).There was statistically significant difference in the length of stay among the high load group,the medium load group and the low load group(P<0.01),yet there was no statistically significant difference between the medium load group and the low load group(P>0.05).There was no significant difference in the duration of mechanical ventilation and number of fever among three groups(P>0.05).After two weeks,the recovery of chest radiography was good,and there was no significant difference among three groups(P>0.05).Conclusion:There is a correlation between the sputum bacterial load of C.trachomatis pneumonia after a certain amount and the clinical characteristics of the child such as time of shortness of breath,duration of fever,severity of chest radiography,and length of hospital stay,but the prognosis is better.