基于肝X受体探讨健脾祛痰法对高脂血症大鼠肝内胆固醇代谢通路的影响

Effect of Spleen-Invigorating and Expectorating Method on Cholesterol Metabolism Pathway in the Liver of Hyperlipidemia Rats Based on Liver X Receptor

目的:基于肝X受体探讨健脾祛痰法对高胆固醇血症的治疗及其代谢通路机制的影响。方法:将40只雄性SD大鼠分成正常组、模型组、中药低、中、高剂量组,正常组正常饮食,模型组给予高脂饲料喂养,各治疗组给予高脂饲料喂养和相应药物[6g/(kg·d)、12g/(kg·d)、25g/(kg·d)],连续进行4周。检测实验相关指标并分析结果。结果:经治疗后,各治疗组肝组织病理学形态有不同程度改善,中、高剂量组大鼠比模型组大鼠血清TC、TG、LDL明显降低,HDL指标明显升高。中、高剂量组MDA水平明显下降,SOD、GSH-PX水平有不同程度的上升。高剂量组LXRαmRNA表达水平显著上升。低、中、高剂量组CYP7A1、ABCG5表达有不同程度的上升。中、高剂量组ABCG8表达水平均增高。结论:健脾祛痰法(温胆汤化裁方)可通过调节肝X受体的表达对CYP7A1、ABCG5和ABCG8的水平进行调节,从而有效减轻肝脏脂肪变性,降低高脂血症大鼠的血脂水平。

Objective:Based on hepatic X receptor to explore the effect of spleen-invigorating and expectorating method on the treatment of hypercholeadiaemia and its metabolic pathway mechanism.Methods:Forty male SD rats were divided into normal group,model group,low-dose,medium-dose,and high-dose Chinese medicine groups.The normal group was fed with a normal diet,the model group was fed with high-fat feed,and each treatment group was fed with high-fat feed and corresponding drugs(6 g/(kg·d),12 g/(kg·d),25 g/(kg·d))for 4 consecutive weeks.Detect relevant indicators of the experiment and analyze the results.Results:After treatment,the histopathological morphology of liver in the treatment groups was improved in different degrees,the serum TC,TG and LDL in the medium-dose and high-dose groups were significantly lower than that in the model group,and the HDL index was significantly higher.The level of MDA decreased significantly in medium and high dose groups,while SOD and GSH-PX increased in different degrees.The mRNA expression level of LXRαin high dose group increased significantly.The expression levels of CYP7A1 and ABCG5 in low-dose,medium-dose and high-dose groups were increased to varying degrees.The expression level of ABCG8 increased in both medium and high dose groups.Conclusion:Spleen-invigorating and expectorating method(Wendan Decoction)can regulate the levels of CYP7A1,ABCG5 and ABCG8 by regulating the expression of liver X receptors,thereby effectively reducing liver steatosis and reducing blood lipid levels in hyperlipidemia rats.